胃癌鸟氨酸脱羧酶基因表达及临床意义  

Expression of ornithine decarboxylase in gastric carcinoma

作　　者：阎云飞[1] 刘斌[1] 徐春晓[1] 辛佳璇[1] 王伟[1] 刘贤锡[1] 

机构地区：[1]山东大学医学院生物化学与分子生物学研究所,济南250012

出　　处：《山东大学学报（医学版）》2009年第3期1-3,共3页Journal of Shandong University:Health Sciences

基　　金：山东省自然科学基金资助项目(z2004c01);教育部博士点自助课题基金资助课题(20040422029)

摘　　要：目的从mRNA和蛋白质水平研究鸟氨酸脱羧酶在胃癌中的表达,探讨其在胃癌形成中的作用。方法采用半定量RT-PCR技术检测胃癌及相应癌旁组织中ODC mRNA的表达量,采用Western-blot在蛋白质水平检测胃癌及相应癌旁组织中ODC的表达量,并做统计学分析。结果21例胃癌组织中ODC在mRNA和蛋白质水平的表达量均高于癌旁组织(P<0.01)。ODC的表达量低分化胃癌组织显著高于高中分化(P<0.01)。结论ODC表达增强在胃癌发生发展中起重要作用,提示ODC可作为胃癌的基因诊断与治疗的靶点。Objective To investigate expression of ornithine decarboxylase （ODC） in both mRNA and protein. Metlaods We extracted the total RNA from 21 surgically removed specimens of gastric cancer and normal mucosa close to lesions and conducted the reverse transcription polymerase chain reaction with optimal condition. Semi-quantitatively we compared the mRNA expression level of ODC between malignant and normal mucosa. Total protein was extracted from the same 21 surgically removed specimens. Western-blot analysis was used to compare the protein expression level of ODC between cancer lesions and normal mucosa. Re- sults mRNA and protein expressions of ODC in lesions of gastric carcinoma were significantly higher than those in contiguous normal mucosa（ P 〈 0.01 ）. Conclusion ODC greatly influences the genesis and development of gastric cancer and may play a pivotal role in the metastasis and infiltration of cancer.

关 键 词：胃肿瘤 鸟氨酸脱羧酶 基因表达 

分 类 号：R735.2[医药卫生—肿瘤] Q786[医药卫生—临床医学]