PDGF-C及其变体PDGF-Cb在肾透明细胞癌中的表达调节及意义  

PDGF-C variant mRNA expression in renal cell carcinoma cells

作　　者：刘娟萍[1] 刘照旭[1,2] 徐忠华 刘承 周尊林 闫磊 范医东 

机构地区：[1]山东大学护理学院,济南250012 [2]齐鲁医院泌尿外科,济南250012

出　　处：《山东大学学报（医学版）》2009年第3期81-84,共4页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金资助课题(30772294/C1612)

摘　　要：目的探讨血小板源性生长因子C(PDGF-C)及其变体血小板源性生长因子Cb(PDGF-Cb)在肾透明细胞癌与相应的正常组织中的表达调节及其意义。方法采用RT-PCR方法检测12例原发性肾透明细胞癌及相对应的正常肾组织中的PDGF-C,并探讨PDGF-C和PDGF-C变体表达的关系。观察顺铂对肾癌细胞株PDGF-C和PDGF-CbmRNA表达的调节作用。结果与其对应的正常组织相比较,12例肾癌组织中,9例PDGF-Cb mRNA表达水平降低,10例全长PDGF-C mRNA表达明显上调,PDGF-C/-Cb的比值有11例明显上升。在mRNA水平,顺铂对PDGF-C及PDGF-Cb呈下调作用,对PDGF-Cb的下调作用呈剂量依赖性。PDGF-C的这两种剪切变体在肾癌组织及与其相应的正常肾组织中表达差异有统计学意义(P<0.05),且全长PDGF-C的表达显著升高。结论PDGF-Cb反向调节PDGF-C的分泌,对PDGF-C表达调节作用可能与肾细胞癌的致癌性相关。顺铂对肾癌细胞株PDGF-C和PDGF-Cb mRNA表达的调节作用为肾癌的致癌机理研究提供了可能的途径。Objective To explore expressions of Platelet-derived growth factor C（PDGF-C） and a novel spliced variant （designated as PDGF-Cb） of human PDGF-C in dear cell renal cell carcinoma and to explore its significance. Methods Using semiquantitative reverse transcription-polymerase chain reaction （RT-PCR） method, expressions of PDGF-C and PDGF-Cb in 12 clear cell renal cell carcinoma tissues and matched adjacent normal tissues were determined. The relationship between expressions of PDGF-C and PDGF-Cb and the impact of Cisplatin on mRNA expression of PDGF-C and PDGF-Cb in the renal cell line was analyzed. Results Compared with the matched normal tissues, 9 of the 12 samples had a decreased PDGF-Cb mRNA expression, 10 had an increased PDGF-C expression, and 11 had an increased PDGF-C/-Cb expression. On the mRNA level, Cisplatin down-regulated expressions of PDGF-C and PDGF-Cb and the effect on the latter was dose-dependently. Expressions of PDGF-C and PDGF-Cb between the carcinoma tissues and the matched normal tissues were significantly different and expression of PDGF-C was up-regulated in the carcinoma tissues. Conclusion PDGF-Cb presents a reverse regulation on the secretion of PDGF-C, and this might be involved in RCC tumorigenesis. The down-regulation of PDGF-C and -Cb induced by Cisplatin provides a potential pathway for research of the tumorigenesis of renal cell carcinoma.

关 键 词：肾肿瘤 顺铂 血小板源性生长因子C 

分 类 号：R737.11[医药卫生—肿瘤]