表柔比星对乳腺癌微球体细胞和单层细胞作用的比较  

The effects of epirubicin on MCF-7 mammosphere cells and monolayer cells:a comparative study

作　　者：黄明主[1] 史亚飞[1] 张凤春[1] 张雁云[2] 

机构地区：[1]上海交通大学医学院附属仁济医院肿瘤科,上海200127 [2]中国科学院上海生命科学研究院上海交通大学医学院健康科学研究所免疫学联合实验室,上海200025

出　　处：《肿瘤》2009年第5期419-422,共4页Tumor

基　　金：国家自然科学基金资助项目(编号:30670798)

摘　　要：目的:探讨表柔比星(epirubicin)对乳腺癌微球体细胞和单层细胞的不同作用。方法:MCF-7细胞在干细胞培养条件下进行微球体培养,MTT法检测表柔比星对MCF-7微球体细胞和单层细胞的抑制率,FCM法检测表柔比星作用下,MCF-7微球体细胞和单层细胞中CD44+CD24-的表达及细胞周期变化。结果:相同质量浓度下,当表柔比星的质量浓度>100ng/mL时,对MCF-7微球体细胞的抑制率明显低于对单层细胞的抑制率(P<0.01);表柔比星(400ng/μL)作用72h后,MCF-7微球体细胞的CD44+CD24-/low比例为(22.8±4.8)%,高于单层细胞的(3.3±0.8)%(P<0.01);MCF-7微球体细胞含有较高比例的G0/G1期细胞(74.33±3.20)%,高于MCF-7单层细胞的(53.40±3.45)%(P<0.01),表柔比星对微球体细胞与单层细胞的G0/G1期影响较小,但显著影响S期及G2期的比例。结论:表柔比星对MCF-7微球体细胞的细胞毒作用较低,并可用于富集乳腺癌干细胞,其对微球体细胞G0/G1比例影响较小。Objective： To explore the different effects of epirubicin on the mammosphere cells and the monolayer cells of breast cancer MCF-7 cells.Methods：MCF-7 cells were cultured in suspension as stem cells to generate primary mammospheres.The inhibitory effects of epirubicin on MCF-7 mammosphere cells and the monolayer cells were measured by MTT assay. The expression of CD44＋CD24- and cell cycle distribution in MCF-7 mammosphere cells and the monolayer cells were analyzed by flow cytometry. Results：When the concentration of epirubicin was higher than 100 ng/mL, the cell inhibition ratio was lower in MCF-7 mammosphere cells than that in the monolayer cells at the same concentration of epirubicin （P〈0.01）. The CD44＋CD24- expression ratio was significantly higher in MCF-7 mammosphere cells than that in the monolayer cells after treatment with 400 ng/μL epirubicin for 72 h, [（22.8±4.8）%] vs [（3.3± 0.8）%] （P〈0.01）. The MCF-7 mammosphere cells had higher proportion of cells in G0/G1 phase than the monolayer cells, [（74.33±3.20）%]vs[（53.40±3.45）%] （P〈0.01）. Epirubicin had little effect on the cells in G0/G1 phase of MCF-7 mammosphere cells and the monolayer cells, but had a great influence on the cell proportion in S and G2 phase. Conclusion： Epirubicin had lower cytotoxicity on MCF-7 mammosphere cells and it can be used to enrich breast cancer stem cells. Epirubicin had little effect on the cell proportion in G0/G1 phase of MCF-7 mammosphere cells as compared with control.

关 键 词：乳腺肿瘤 肿瘤干细胞 表柔比星 细胞培养技术 

分 类 号：R73-354[医药卫生—肿瘤] R737.9[医药卫生—临床医学]