人宫颈癌组织CDH1和PAX1基因甲基化研究  

Study on the methylation status of CDH1 and PAX1 genes in human cervical carcinoma tissues

作　　者：徐军[1] 王红琳[1] 陆杲川[1] 林晓[1] 

机构地区：[1]上海市第八人民医院妇产科,上海200233

出　　处：《肿瘤》2009年第5期483-485,共3页Tumor

基　　金：上海市卫生局科研课题计划资助项目(编号:2006122)

摘　　要：目的:检测上皮型钙黏附素(E-cadherin,CDH1)基因和配对盒基因家族PAX1基因在宫颈癌组织及人乳头瘤病毒(human papillom avirus,HPV)感染的正常宫颈组织、宫颈炎组织、宫颈上皮内瘤样病变(cervical intraepithelial neoplasia,CIN)Ⅰ组织、CINⅡ～Ⅲ组织和宫颈癌组织中的甲基化情况,评估是否可将其作为临床诊断的分子标志物。方法:应用甲基化特异性聚合酶链反应法(methylation specific PCR,MSP)对HPV感染的正常宫颈组织、宫颈炎组织、CINⅠ组织、CINⅡ～Ⅲ组织以及宫颈癌组织中的CDH1和PAX1基因进行甲基化检测。结果:HPV感染的正常宫颈组织、宫颈炎组织和CINⅠ组织中未检出CDH1基因甲基化;CINⅡ～Ⅲ组织中检出CDH1基因甲基化2例(13.3%),宫颈癌组织中检出CDH1基因甲基化9例(22.5%),与HPV感染的正常宫颈组织比较差异均有统计学意义(P<0.05)。HPV感染的正常宫颈组织和宫颈炎组织中未检出PAX1基因甲基化,CINⅠ、CINⅡ～Ⅲ和宫颈癌组织的PAX1基因甲基化阳性率分别为13.3%、46.7%和87.5%,CINⅡ～Ⅲ组织与CINⅠ和宫颈癌组织比较差异均有统计学意义(P<0.05)。CINⅠ组织、CINⅡ～Ⅲ组织和宫颈癌组织的CDH1和PAX1基因甲基化总阳性率分别为13.3%、60.0%和97.5%。结论:CDH1和PAX1基因甲基化,尤其是PAX1基因甲基化对于宫颈癌临床诊断具有潜在价值。Objective： To determine the methylation status of E-cadherin （CDH1） and PAX1 gene, the member of paired box family, in cervical carcinoma tissues, human papillomavirus （HPV）-infected normal cervical tissues, cervicitis tissues, cervical intraepithelial neoplasia （CIN） Ⅰ tissues, and CIN Ⅱ-Ⅲ tissues, and assess whether changes in methylation status can be used as a mole-cular marker in clinic diagnosis. Methods：Methylation status of CDH1 and PAX1 gene was detected using methylation-specific PCR （MSP） in HPV-infected normal cervical tissues （n=15）, cervicitis tissues （n=15）, CINⅠ tissues （n=15）, CINⅡ Ⅲ tissues （n=15）, and cervical carcinoma （n=40） tissues. Results： （1） CDH1 methylation was undetected in HPV infected normal cervical tissues, cervicitis and CINⅠspecimens, while it was frequently observed in two CINⅡ-Ⅲ specimens and nine cervical carcinoma specimens （13.3% and 22.5%, respectively）. The difference was significant compared with HPV-infected normal cervical tissues （P〈0.05）. （2） PAX1 methylation was not observed in HPV-infected normal cervical tissues and cervicitis specimens. The positivity of methylation was 13.3%, 46.7%, and 87.5% in CINⅠ, CIN Ⅱ Ⅲ, and cervical carcinoma specimens, respectively. The difference was significant between CIN Ⅰ and CIN Ⅱ - Ⅲ specimens and cervical carcinoma specimens （P〈0.05）. （3） Hypermethylation was more frequent in CIN Ⅱ-Ⅲ than in CIN Ⅰ specimens （60.0% vs 13.5%, P〈0.05） as well as in cervical carcinoma than in CIN Ⅱ-Ⅲ specimens （97.5% vs 60.0%, P〈0.05）. Conclusion： Methylation of CDH1 and PAX1 gene, especially PAX1 gene, may have a potential value for diagnosis of cervical carcinoma in clinic.

关 键 词：宫颈肿瘤 DNA甲基化 基因 CDH1 基因 PAX1 

分 类 号：R737.33[医药卫生—肿瘤]