内皮素1对喉癌细胞HEP 2的促增殖作用  

Effect of endothelin 1 on mitosis of human HEP 2 cells in vitro

作　　者：徐定远[1] 郑雪莲[2] 吴波[3] 薛飞[1] 李泽卿[1] 季俊峰[1] 王秋萍[1] 

机构地区：[1]南京军区总医院耳鼻咽喉科,南京210002 [2]四川大学华西第二附属医院分子与转化实验室,成都610041 [3]南京军区总医院病理科,南京210002

出　　处：《山东大学耳鼻喉眼学报》2009年第2期1-4,共4页Journal of Otolaryngology and Ophthalmology of Shandong University

基　　金：中国博士后基金研究项目(20070421033);四川省科技厅应用基础研究项目部分资助(2008JY0026)

摘　　要：目的探讨内皮素1对HEP 2细胞有丝分裂的影响。方法采用RT-PCR及免疫组化Dako EnVision法检测HEP 2细胞内皮素受体表达情况;3H-胸腺嘧啶核苷渗入法检测不同浓度和作用时间内皮素1对HEP 2细胞有丝分裂的影响。结果HEP 2细胞存在内皮素受体基因及蛋白表达;内皮素1具有明显促HEP2细胞增殖作用,且其促增殖作用呈浓度依赖性。结论内皮素1能有效促进HEP 2细胞有丝分裂,对喉癌的演进可能有重要调控作用。Objective To investigate the effect of endothelin 1 on mitosis of HEP 2 cells. Methods Expression of endothelin receptors on HEP 2 cells was determined by RT-PCR and immunohistochemical methods HEP 2 cell hnes were exposed to different concentrations of the endothelin 1, and mitosis of HEP 2 cells was measured by 3 H-TdR at different time points. Results Gene expression of the endothehn receptor was detected by RT-PCR studies showed that endothelin receptor immunoreactivity appeared in HEP 2 cells. Addition of endothelln 1 resulted in an increase of DNA synthesis in concentration dependence. Conclusion Endothelin 1 can promote mitosis of HEP 2 cells and may play an important role in the progression of laryngeal carcinoma.

关 键 词：内皮素1 HEP2细胞 有丝分裂 

分 类 号：R767.19[医药卫生—耳鼻咽喉科]