颈段食管癌患者外周血单个核细胞HLA-A和HLA-B mRNA的表达  

Expressions of HLA-A and B mRNA in peripheral blood mononuclear cells in patients with esophagus cancer of the neck

作　　者：李浩[1] 邹雄[2] 赵胜梅[1] 张义[2] 李英杰[1] 陆楠[1] 周玉侠[1] 竺明晨[1] 刘阳[1] 

机构地区：[1]山东大学医学院,济南250012 [2]山东大学齐鲁医院检验科,济南250012

出　　处：《山东大学耳鼻喉眼学报》2009年第2期8-11,共4页Journal of Otolaryngology and Ophthalmology of Shandong University

基　　金：山东省科技厅国际合作项目(2005GG3202025)

摘　　要：目的检测颈段食管癌患者外周血单个核细胞(peripheral blood mononuclear cell,PBMC)HLA-A和HLA-B mR-NA的表达水平,探讨其在肿瘤免疫中的作用及其临床应用价值。方法收集32例颈段食管癌患者和45名健康体检者外周血单个核细胞,用实时荧光RT-PCR方法检测淋巴细胞HLA-A和HLA-B mRNA的表达水平。结果PBMC的HLA-A和HLA-B mRNA在颈段食管肿瘤组的相对表达率分别为0.41±0.26和0.37±0.28,在正常对照组分别为1.12±0.54,1.19±0.60,颈段食管肿瘤组HLA-A和HLA-B mRNA相对表达率低于正常对照组(P均<0.000 1)。多因素Logistic回归分析显示,HLA-A,B mRNA的表达与颈段食管癌的发生有关(P=0.01)。结论颈段食管癌患者外周血单个核细胞HLA-A和HLA-B mRNA表达水平低于正常对照组。HLA-A和HLA-B mRNA的表达水平或许可以作为一个新的肿瘤标志物,这对颈段食管癌的早期诊断和治疗提供了一个新的思路和方向。Objective＇ To investigate expression levels of HLA-A and B mRNA in peripheral blood mononuclear ceils （PBMCs） in patients with esophagus cancer of the neck and to explore their clinical significance. Methods Peripheral blood mononuclear cells HLA-A and B mRNA were determined by a real-time reverse quantitative PCR method in 62 patients with esophagus cancer of the neck and 32 healthy individuals. Results The relative expression ratio of PBMC I-ILA-A and B mRNA was 0.41 ± 0.26 and 0.37 ±0.28 in the carcinoma group and 1.12 ± 0.54 and 1.19 ± 0.60 in the control group. Expression levels of HLA-A and B mRNA on PBMCs in esophagus cancer of the neck were significantly lower than that in the controls. Multiple logistic regression analysis showed that expression of HLA-A, B was significantly associated with esophagus cancer of the neck（ P = 0. 01 ）. Condusion HLA-A and B mRNA play a critical role in the tumor immunity response. HLA-A and B mRNA expression levels in PBMC axe significantly reduced when tumors occur, so they may become available tumor markers.

关 键 词：颈段食管癌 外周血单个核细胞 HLA 实时荧光定量PCR 

分 类 号：R735.1[医药卫生—肿瘤]