龙眼花芽与叶芽差异蛋白分析  

Analysis of Differential Proteins during Development Stages of Flower Bud and Leaf Bud in Longan

作　　者：庞国彩[1,2] 谢东丽[1,2] 陈清西[1,3] 陈伟[1,2] 龙强[1,2] 

机构地区：[1]福建农林大学,作物遗传育种与综合利用教育部重点实验室,福州350002 [2]福建农林大学,生命科学学院,福州350002 [3]福建农林大学,园艺学院,福州350002

出　　处：《热带亚热带植物学报》2009年第3期242-248,共7页Journal of Tropical and Subtropical Botany

基　　金：国家自然科学基金项目(30571293);教育部博士点基金项目(200803890009);福建省自然科学基金项目(B0710006);福建省教育厅科技计划项目(JA05236)资助

摘　　要：以龙眼(Dimocarpus longan Lour.)花芽和叶芽为材料,对龙眼花芽与叶芽分化发育过程中的差异蛋白质进行分析和鉴定,并探讨其生物学功能。本研究共鉴定出10个差异蛋白质:ATP synthase beta subunit,fructokinase,LHCII type I chlorophyll a/b binding protein,peroxidase,ascorbate peroxidase,14-3-3 protein,14-3-3 family protein,putative cytosolic cysteine synthase 7,retrotransposon protein,putative,Ty1-copia subclass,Protein Group similar to lateembryogenesis abundant proteins。这些差异蛋白质可能与龙眼花芽与叶芽的物质和能量代谢、自由基清除和抗氧化作用、信号转导和基础代谢、氨基酸代谢等生理过程密切相关。The proteomes during development stages of flower bud and leaf bud in longan （Dimocarpus longan Lour.） were isolated and identified by using 2-DE technology and MS analysis. Ten proteins were identified by MALDI-TOF-TOF/MS, such as ATP synthase beta subunit, fructokinase, LHCⅡ type Ⅰ chlorophyⅡ a/b binding protein, peroxidase, ascorbate peroxidase, 14-3-3 protein, 14-3-3 family protein, putative cytosolic cysteine synthase 7, retrotransposon protein, putative, Tyl-copia subclass, protein group similar to late embryogenesis abundant proteins. These proteins might involve in substance and energy metabolism, scavenging and anti- oxidation of free radical, signal transduction and basic metabolism, amino acid metabolism and other physiological processes.

关 键 词：龙眼 花芽 叶芽 蛋白质组 双向电泳 质谱 

分 类 号：S667.2[农业科学—果树学]