人子宫肌瘤上皮细胞的分离培养及GnRH I在其中的表达与意义  

Expression and Significance of GnRH I in Epithelium Cells After Separating and Culturing from Endometrial Tissue in Vitro

作　　者：曾伟前 汪文玉[1] 王燕[1] 

机构地区：[1]湖南省娄底市中心医院,湖南娄底417000

出　　处：《实用预防医学》2009年第3期916-918,共3页Practical Preventive Medicine

摘　　要：目的分离培养人子宫内膜上皮细胞,初步探讨GnRH I在其中的表达与意义。方法分离正常人子宫内膜上皮细胞与子宫肌瘤患者子宫内膜上皮细胞并传代培养,采用角蛋白单克隆抗体进行鉴定后,免疫组化检测GnRH I蛋白的表达,流式细胞术检测GnRH I对子宫内膜上皮细胞凋亡的影响。结果分离的子宫内膜上皮细胞在体外能够生长良好并能稳定传代,GnRH I在正常子宫内膜上皮细胞不表达或弱表达,在子宫肌瘤患者子宫内膜上皮细胞中高表达或表达增强;流式细胞术检测提示在人子宫肌瘤上皮细胞发生的凋亡率高于正常子宫内膜上皮细胞,两者比较差异有统计学意义。结论体外分离培养子宫内膜上皮细胞成功;GnRH I在正常子宫上皮细胞与子宫肌瘤上皮中存在差异表达;GnRH I蛋白能够引起子宫肌瘤上皮细胞发生凋亡,可能通过信号传导通路参与了子宫肌瘤的发生与发展。Objective To approach the expression and significance of GnRH I in epithelium cells after separating and culturing from endometrial tissue in vitro. Methods The epithelium cells from endometrial tissue of the healthy adults and the uterine fibroids sufferers were separated and cultured. The cells were identified by monoclonal antibody of keratin. The GnRH I protein was detected by immunohistochemisty. And the influence of GnRH I to apoptosis of epithelium was determined by flow cytometry. Results The epithelium cells could grow well in vitro and passage stably in vitro. There was a signifi- cant difference that GnRH I was not or weakly express in normal endometrial cell, but was highly expressed in uterine fibroids cells. The apoptotic rate in uterine fibroids cells was higher than that in the normal endometrial cells by the detection method of flow secytometry. Conclusions The separation and culture of endometrial epithelial cell in vitro is successfully. The ex- pression has a significant difference between normal endometrial cell and uterine fibroids cell. GnRH I protein can cause endometrial epithelial cell into apoptosis, and participate the signal transduction in the development of the uterine fibroids possibly.

关 键 词：子宫内膜上皮细胞 分离培养 表达 凋亡 

分 类 号：R737.33[医药卫生—肿瘤]