Myostatin基因5′调控区的多态与猪生长性能的关系  

Relationship between Polymorphism of 5′Regulatory Region of Porcine MSTN and Growth Performance

作　　者：吴俊红[1] 武艳群[1] 赵晓枫[1] 吴旧生[1] 徐宁迎[1] 

机构地区：[1]浙江大学华家池校区动物科学学院,杭州310029

出　　处：《畜牧兽医学报》2009年第5期617-621,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家重大基础规划项目(2006CB102101);国家高技术发展计划项目(2006AA10Z1A2)

摘　　要：采用PCR-RFLP技术对45头金皮(金华猪×皮特兰猪)F2代猪Myostatin基因5′调控区的多态性进行检测,根据酶切结果将其分为AA、TT和AT 3种基因型,结果显示A等位基因频率为0.467,T等位基因频率为0.533。对这些个体进行生长和屠宰性能测定以及对背最长肌进行免疫组化分析,进而采用SPSS程序分析Myo-statin不同基因型对金皮F2代猪生长性状、肌纤维组织学特性及胴体性状的影响。结果表明:Myostatin基因对金皮F2代猪肌纤维直径、MHC I型肌纤维比例、肩部背膘厚、眼肌肉色亮度及出生至4月龄平均日增质量的影响均达到显著水平(P<0.05)。The polymorphism of the 5′regulatory region of Myostatin gene was detected using PCR-RFLP method in 45 Jinpi F2 pigs. The results showed that there were 3 genotypes （AA, TT and AT） and their frequencies were 0. 244, 0. 311 and 0. 444, respectively. Growth, carcass traits and Longissimus dorsi muscles analyzed using immunohistochemical were recorded. The resuits showed that the Dra I polymorphism of MSTN gene had significant effect（P〈0.05） on the muscle fiber diameter, the percentage of MHC type Ⅰ fiber, the backfat thickness （Shoulder） ,the average daily gain from birth to 4 months old and the eye muscle lightness.

关 键 词：金皮F2代猪 MYOSTATIN 肌纤维组织学性状 生长性能 胴体性状 

分 类 号：S828[农业科学—畜牧学] Q343.15[农业科学—畜牧兽医]