表达传染性支气管炎S1基因和IFN-γ基因重组鸡痘病毒安全性与最适免疫剂量分析  

Safety Evaluation and Minimum Immune-doses Analysis of the Recombinant Fowl Pox Virus Expressing S1 Gene of Infectious Bronchitis Virus and IFN-γ Gene

作　　者：石星明[1] 王云峰[1] 王玫[1] 兰德松[1] 任刚[2] 李继松[1] 曾伟伟[1] 孙妍[1] 刘胜旺[1] 童光志[3] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/禽传染病研究室,哈尔滨150001 [2]东莞出入境检验检疫局,东莞523071 [3]中国农业科学院上海兽医研究所,上海200232

出　　处：《畜牧兽医学报》2009年第5期712-716,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家“863”计划资助项目(2003AA213020)

摘　　要：对共表达鸡传染性支气管炎病毒S1基因和II型干扰素基因的重组鸡痘病毒（rFPV-IFNγS1）冻干疫苗进行安全性和最适免疫剂量的研究。取冻干疫苗，加生理盐水恢复至冻干前体积，分别取0.1 mL经翅内侧无血管处皮下接种1周龄SPF鸡，接种剂量为1.0×10^5PFU，接种后3 d出现局部反应，5 d局部肿胀达到最大，接种鸡无其它全身反应，精神、食欲以及发育等均不受影响，说明重组病毒对实验鸡是安全的。将重组疫苗用生理盐水作倍比稀释后翅内侧无血管处皮下接种4周龄SPF鸡，接种剂量为1.0×10^1-1.0×10^4PFU，免疫后3周攻击传染性支气管炎病毒LX4株。结果表明，在接种剂量为1.0×10^2-1.0×10^4PFU的剂量范围内均可以使全部实验鸡产生局部反应，并对IBV的攻击形成保护，降低免疫鸡的发病率和死亡率，发病保护率达到73%以上；1.0×10^1PFU接种实验鸡后，仅有5/11的鸡出现局部反应，对IBV攻击的发病保护率为64%，与对照组差异不显著（P〉0.05）。以上结果说明，疫苗接种后的局部反应与重组疫苗的免疫效力之间具有相关性，根据疫苗生产使用实际情况确定传染性支气管炎重组鸡痘病毒疫苗的最适免疫剂量为10^3PFU。This experiment was conducted to study the safety evaluation and minimum immunedoses of the recombinant fowl pox virus expressing S1 gene of infectious bronchitis virus and IFN-γ gene. The vaccines were chosen and diluted with the saline water. 7-day-old SPF chickens were inoculated in the endothelium under the wing at the dose of 1.0 × 10^5PFU per chicken, local reaction appeared three days later, local swell became the largest at the 5th day. The inoculated chicken showed no sign of other discomforts, and inspirit, appetite and development were the same as controls. The results indicated that the vaccine was safe for chicken. The vaccine was diluted to 1.0 × 10^1-1.0 × 10^4 PFU, and the 28-day-old SPF chickens were inoculated as above, then challenged with infectious bronchitis virus LX4 strain 3 weeks after inoculation. The results showed that the chickens inoculated in the dose range of 1.0 × 10^2 to 1.0 × 10^4PFU could induce local reaction, morbidity and mortality was reduced, and the protection rate was exceed 73%, but the inoculum of 1.0 × 10^1 PFU can induce local reaction partially （5/11）, and the protection rate was 64%. These results indicated that there was a correlation between the local reaction and the efficiency, and the local reaction can reflect the efficiency of the vaccine. According to the fact of vaccine production and use, the optimum dose of the vaccine was 10^3 PFU.

关 键 词：传染性支气管炎 重组鸡痘病毒 S1基因 最适免疫剂量 

分 类 号：S852.4[农业科学—基础兽医学] S858.315.3[农业科学—兽医学]