正畸力对幼鼠牙本质中DSP表达的影响  

The effects of orthodontic force on DSP expression in dentine of young rats

作　　者：孔祥伟[1] 曹猛[1] 丁寅[1] 刘晓东[2] 

机构地区：[1]西安第四军医大学口腔医院正畸科,710032 [2]西安第四军医大学口腔医院解剖生理教研室,710032

出　　处：《实用口腔医学杂志》2009年第3期323-326,共4页Journal of Practical Stomatology

摘　　要：目的:探讨正畸力对牙齿发育过程的牙本质中DSP表达的影响。方法:选取5周龄雄性SD大鼠,建立正畸牙齿移动模型。分别于加力后1、3、7、14、21d处死动物,取含双侧上颌第一磨牙的上颌标本,制作石蜡切片,进行HE染色和DSP免疫组化染色。结果:牙冠部牙本质小管、前期牙本质、成牙本质细胞和牙根部成牙本质细胞DSP染色阳性。其中,牙尖部位的牙本质小管和前期牙本质呈强阳性染色,14d时达到高峰,21d时阳性染色有所减弱;根部成牙本质细胞在实验初期呈弱阳性染色,随时间的延长阳性染色逐渐增强。正常对照组DSP的表达明显弱于实验组。结论:对处于发育晚期的年轻恒牙施加适当的正畸力会促使成牙本质细胞进入活跃状态,牙本质中DSP表达上调,从而在一定程度上加速牙本质的形成及矿化。Objective： To investigate the effects of orthodontic force on DSP expression in dentine during rat teeth development. Methods ： Standardized orthodontic teeth movement was performed in six groups of SD male rats aged 5 weeks. Animals were sacrificed for histological investigation at 1, 3, 7, 14 and 21 days after orthodontic teeth movement. Immunohistochemisical staining revealed the distribution and expression of DSP in maxillary first molar during orthodontic teeth movement. Results： Expressions of DSP were detected in dentinal tubules, predentin and odontoblasts, especially at the cusp area. Immunoreactions for DSP in crown dentine were strongest at the 14th day, but relatively weak at the 21st day. Immunoreactions for DSP in root dentine were weaker than those in the crown, and increased in a time dependent manner. Statistical analysis showed that the immunoreactions of DSP in the experimental group were significantly stronger than in normal control rats. Conclusion： At later development stages of immature permanent teeth, moderate orthodontic force may promote the activation of odontoblasts and increase the expression of DSP in dentine, thereby accelerating the dentine formation and mineralization.

关 键 词：正畸学 牙本质形成 牙本质涎蛋白 

分 类 号：R783.5[医药卫生—口腔医学]