涎腺腺样囊性癌耐药细胞系的建立  

Establishment of a multidrug resistant human salivary gland adenoid cystic carcinoma cell line

作　　者：秦楠[1] 缪叶[1] 李焰[1] 刘峰[1] 吴军正[1] 

机构地区：[1]西安第四军医大学口腔医学院,710032

出　　处：《实用口腔医学杂志》2009年第3期403-407,共5页Journal of Practical Stomatology

基　　金：国家自然科学基金项目(编号:30672343)

摘　　要：目的:建立博莱霉素(BLM)诱导的人涎腺腺样囊性癌耐药细胞株。方法:采用大剂量博莱霉素(20μ/ml)24h暴露法反复处理腺样囊性癌细胞系ACC-2细胞,获得耐药细胞系ACC-2/BLM;通过MTT法、细胞计数法、流式细胞术等方法,观察ACC-2/BLM的生物学特性。结果:成功建立了博莱霉素诱导的人涎腺腺样囊性癌耐药细胞系ACC-2/BLM,耐药指数为7.299,与环磷酰胺、顺铂等抗癌药有明显的交叉耐药;与ACC-2细胞相比,ACC-2/BLM倍增时间延长,细胞周期中S期细胞减少,G1、G2期细胞增多。在含60μg/ml博莱霉素的培养液中,分别培养9d后,ACC-2/BLM凋亡细胞明显少于ACC-2细胞。结论:BLM可致ACC-2细胞产生耐药性,ACC-2/BLM细胞系具有多药耐药特性。Objective： To establish a muhidrug resistant human salivary gland adenoid cystic carcinoma cell line. Methods： Human salivary gland adenoid cystic carcinoma cell line ACC-2 was treated by 24-hour-exposure to high dose of Bleomycin（BLM） （20 μg/ml）. Drug sensitivity was evaluated by MTT assay. Cell counting was employed to make the growth curve and to calculate the cell doubling time. Flow eytometry （FCM） was used to study the cell cycle distribution and apoptosis. The colony formation ability was also observed. Results： Multidrug resistant cell line of human salivary gland adenoid cystic carcinoma was established and named ACC-2/BLM. After 10 times repeated exposure to BLM, the resistance index （RI） to BLM, 5- Fluorouracil （ 5- FU） , Cisplatin （CDDP） , Cyclophosphamide（CTX） , Vincristine（VCR） were 7. 299, 1.03, 2.15, 1. 114 and 5.96 respectively. Compared with ACC-2, the proliferation potential of ACC-2/BLM cells was decreased. The ACC-2 apoptosis cells were much more than ACC-2/BLM cells after 9-day-treatment by BLM at 60 μg/ml. Conclusion： ACC-2/BLM cell line has multidrug resistant characteristics.

关 键 词：腺样囊性癌 多药耐药性 博莱霉素 凋亡 

分 类 号：R739.8[医药卫生—肿瘤]