血管利钠肽对大鼠肺动脉的舒张作用及机理  

Vasorelaxing effect of vasonatrin peptide on pulmonary artery in rats

作　　者：冯华松[1,2,3] 臧益民[1,2,3] 朱妙章[1,2,3] 裴建明[1,2,3] 王跃民[1,2,3] 牛国保[1,2,3] 王琳[1,2,3] 施溥涛 

机构地区：[1]第四军医大学生理学教研室 [2]第四军医大学唐都医院呼吸内科 [3]上海生物化学研究所

出　　处：《心功能杂志》1998年第2期69-71,共3页

基　　金：国家自然科学基金

摘　　要：血管利钠肽（ＶＮＰ）是新近人工合成的由２７个氨基酸残基组成的肽类物质，它是心房利钠利尿肽（ＡＮＰ）和Ｃ型利钠利尿肽（ＣＮＰ）的杂交物。我们采用离体血管灌流的方法观察了ＶＮＰ对离体大鼠肺动脉和腹主动脉的舒张作用。表明ＶＮＰ对内皮完整的与去内皮的肺动脉和腹主动脉均有浓度依赖性舒张作用，其对抗去甲肾上腺素（１０μｍｏｌ／Ｌ）或ＫＣｌ（６０ｍｍｏｌ／Ｌ）收缩肺动脉的最大舒张效应明显大于腹主动脉；内皮完整和去内皮组之间无显著性差异；在浴槽内预先加入优降糖可显著减低肺动脉和腹主动脉对ＶＮＰ的舒血管效应，预先加入心得安则不明显影响ＶＮＰ对肺动脉和腹主动脉的舒血管作用。提示ＶＮＰ对上述血管的舒张作用不依赖于内皮的存在。Vasonatrin peptide(VNP) is a newly synthesized 27 amino acid peptide, which is a chimera of atrial natriuretic peptide(ANP) and C type natriuretic peptide(CNP). In the present study, the vasorelaxing effect of VNP on rat pulmonary artery was investigated by in vitro perfusion, in contrast to the abdominal aorta. The results showed that VNP caused concentration dependent relaxations in isolated rat pulmonary artery and abdominal aorta with and without endothelium. The maximal relaxation of VNP to reverse 60 mmol/L KCl or 10 μmol/L norepinephrine induced contraction was significantly greater in pulmonary artery than in abdominal aorta. The vasodilating actions induced by VNP, were significantly attenuated in organ chambers of pre incubation with glybenzcy clamide, and not changed in organ chambers of pre incubation with propranolol. These results suggest that the diastolic effect of VNP on the above mentioned blood vessels is independent on endothelium and is also modulated by blockers of ATP sensitive K + channels.

关 键 词：血管利钠肽 肺动脉 主动脉 大鼠 药理 

分 类 号：R972[医药卫生—药品] R965.2[医药卫生—药学]