多聚酶链反应检测胃粘膜幽门螺杆菌  

Detection of Helicobacter pylori in gastric biopsy by polymerase chain reaction

作　　者：华杰松[1,2] 郑鹏远[1,2] 

机构地区：[1]新加坡国立大学医学院微生物学系 [2]法国Bordeaux第二大学细菌学系

出　　处：《华人消化杂志》1998年第5期377-379,共3页

摘　　要：目的采用ＰＣＲ技术检测Ｈｐ．方法８３例胃病患者粘膜标本经多聚酶链反应，组织学及快速尿素酶试验进行分析．结果在８２例患者中，多聚酶链反应阳性３２例（３８６％），组织病理切片阳性２９例（３４９％），快速尿素酶试验阳性２８例（３３７％）．三种方法无统计学差异．存放于尿素酶试剂中的胃粘膜标本不影响多聚酶链反应结果．结论多聚酶链反应是检测Ｈｐ的一种敏感性及特异性较强的方法．在将标本送经实验室时无需特殊的运输条件．AIM To detect Helicobacter pylori (Hp) in gastric biopsy by using PCR. METHODS Gastric specimens from 83 patients with gastric diseases were analyzed by histology, rapid urease test and polymerase chain reaction using primers based on specific Hp M r 26000 protein genes. RESULTS Out of 83 patients tested, 32 (38 6%) were Hp positive by PCR, while 29 (34 9%) and 28 (33 7%) cases were positive by histology and rapid urease test, respectively. There were no statistical differences among the 3 tests. It was noted that transportation of gastric specimen placed in urease test reagent did not affect polymerase chain reaction. CONCLUSION Polymerase chain reaction is a sensitive and specific method to detect Hp. It needs no special transportation conditions to send gastric biopsy to laboratory.

关 键 词：胃粘膜 幽门螺杆菌 分离 提纯 PCR 

分 类 号：R573[医药卫生—消化系统]