siRNA对肝癌细胞异常表达FAT10基因的抑制效应研究  

Study on inhibitory effect of small interfering RNA on abnormal expression of FAT10 in hepatoma carcinoma cell

作　　者：刘天德[1] 余新[1] 洪波[1] 邵江华[1] 

机构地区：[1]南昌大学第二附属医院肝胆外科江西省分子医学重点实验室,330006

出　　处：《新医学》2009年第6期358-360,417,共4页Journal of New Medicine

基　　金：2008年度教育部科学技术研究重点项目(208070);2008年江西省教育厅科学技术研究重点项目(GJJ08003);2007年南昌大学研究生创新基金项目资助(YC07B007)

摘　　要：目的:探讨小干扰RNA(small interfering RNA,siRNA)对原发性肝癌(肝癌)细胞中异常表达的类泛素蛋白FAT10的抑制作用,研究其对肝癌细胞生长的影响。方法:针对FAT10基因设计4条siRNA序列,并转染至人肝癌细胞株Hep3B细胞,通过实时荧光定量PCR检测siRNA对FAT10基因表达的影响,使用四甲基偶氮唑蓝法检测siRNA对Hep3B细胞生长情况的影响,采用流式细胞术分析siRNA对Hep3B细胞周期变化的影响。结果:Hep3B细胞转染siRNA后,FAT10 mRNA表达降低,Hep3B细胞的生长受到了明显的抑制,其生长主要停滞在G0/G1期,而S期和G2/M期细胞所占比例下降。结论:siRNA可抑制Hep3B细胞FAT10基因的表达,并抑制Hep3B细胞的生长。FAT10基因可能是调控肝癌基因表达的新的靶基因。Objective： To explore the inhibition effect of small expression of ubiquitin-related protein FAT10 in hepatoma carcinoma cell on the growth of HCC. Methods： Four siRNA sequences were designed interfering RNA （siRNA） on abnormal （HCC）, and thus to study its influence and transfected into Hep3B cells. The tendency of FAT10 expression was determined using real time fluorescent quantitation -polymerase chain reaction. The effect on cell growth was detected by MTY and the effect on cell cycle was analyzed by flow cytometry when FAT10 was degraded by siRNA. Results： The cell growth ability of Hep3B ceils was significantly inhibited following degradation of FAT10 mRNA by siRNA transfection. Hep3B cells were mainly detained in G0/G1 and the percentage of S and G2/M decreased. Conclusion： siRNA can effectively inhibit abnormal expression of FAT10 as well as the growth of Hep3B. FAT10 may be a novel target in gene regulation of hepatocellular carcinoma.

关 键 词：肝细胞癌 原发性 肝细胞 类泛素蛋白 小干扰核糖核酸基因 肿瘤抑制 

分 类 号：R735.7[医药卫生—肿瘤] R394[医药卫生—临床医学]