白细胞介素18和基质金属蛋白酶9在人冠状动脉粥样硬化斑块中的表达  

Expression of Interleukin-18 and Matrixmetalloproteinase-9 in Atherosclerosis Plaque of Human Coronary Artery

作　　者：郭静[1] 于霄[2] 孙雷[2] 

机构地区：[1]抚顺矿务局总医院病理科,辽宁省抚顺市113008 [2]大连医科大学病理教研室,辽宁省大连市116027

出　　处：《中国动脉硬化杂志》2009年第4期285-288,共4页Chinese Journal of Arteriosclerosis

摘　　要：目的探讨白细胞介素18和基质金属蛋白酶9在不同程度的人冠状动脉病变中的表达情况及意义。方法收集14例尸检的人冠状动脉共48个冠状动脉标本,根据HE染色将标本分为不稳定斑块组、稳定斑块组和正常对照组。用免疫组织化学及逆转录聚合酶链反应检测白细胞介素18和基质金属蛋白酶9蛋白及mRNA在以上不同类型标本中的分布表达。结果白细胞介素18和基质金属蛋白酶9表达于冠状动脉粥样硬化斑块区,在不稳定斑块中的表达明显高于稳定斑块。并且二者的表达强度呈正相关。结论白细胞介素18和基质金属蛋白酶9可能参与冠状动脉粥样硬化斑块的发生、发展,与斑块不稳定性密切相关。并且二者协同参与粥样斑块发展。Aim To investigate the expression and significance of interleukin-18 and matrixmetalloproteinase-9 in human coronary arteries with different atherosclerotic lesions. Methods 48 coronary arteries specimens from 14 autopsy cases were divided into 3 different groups according to HE staining, which were unstable plague group, stable plague group and normal group. These specimens were detected by immnnohistochemistry and reverse transcription-polymerase chain reaction for protein and mRNA analysis. Results Interleukin-18 and matrixmetalloproteinase-9 mRNA and pro- tein expression can be detected in atherosclerosis, and there is significant difference between unstable plaques and stable plaques. The levels are significantly higher in unstable plaques. Positive association between intedeukin-18 and matrix- metalloproteinase-9 expression intensity showed in Spearman tests. Conclusions These results indicates interleukin- 18 and matrixmetalloproteinase-9 both participate in the initiation and development of atherosclerosis and are highly relevant to the unstable plaque. These findings suggests that interleukin-18 is in coordination with matrixmetalloproteinase-9 during the development of atberosclerosis.

关 键 词：冠状动脉粥样硬化 斑块 白细胞介素18 基质金属蛋白酶9 免疫组织化学 逆转录聚合酶 链反应 

分 类 号：R36[医药卫生—病理学]