凋亡相关基因Livin、Smac/DIABLO在胃癌组织中的表达及其意义  

Expression and clinical significance of apoptosis associated genes Livin and Smac /DIABLO in human gastric carcinomas

作　　者：赵毅[1] 邓鑫[1] 王强[1] 

机构地区：[1]中国医科大学附属盛京医院胃肠外科,沈阳辽宁110004

出　　处：《癌症》2009年第6期593-601,共9页Chinese Journal of Cancer

基　　金：辽宁省自然科学基金项目(No.20072109);沈阳市自然科学基金项目(No.1071200-1-00)~~

摘　　要：背景与目的:作为一种促凋亡基因,Smac/DIABLO与凋亡抑制基因Livin相互作用,共同参与调控细胞癌变的起始及发展。本研究检测凋亡抑制蛋白Livin及线粒体促凋亡蛋白Smac/DIABLO在胃癌组织中的表达,观察其表达水平与胃癌各临床病理因素的关系。方法:应用实时荧光定量PCR SYBR Green荧光染料法检测75例手术切除的胃癌组织、20例癌旁组织及正常胃组织中Livin mRNA和Smac/DIABLO mRNA的表达,Western blot结合及免疫组化SP法检测两种蛋白的表达及组织学定位。结果:正常胃组织及癌旁组织中均无Livin mRNA表达,胃癌组织中Livin mRNA表达显著上调,相对表达量为6.374±4.759,低分化胃腺癌组织及有淋巴结转移组的相对表达量显著高于中高分化胃腺癌组织(P<0.05),其表达与肿瘤大小、浸润深度及TNM分期无关,Livin蛋白的表达结果与之相符;胃癌组织中Smac/DIABLO mRNA的表达(0.731±0.420)低于正常胃组织(1.104±0.276)及癌旁组织(1.061±0.737),但差异无统计学意义(P>0.05),Smac/DIABLO mRNA表达水平与胃癌各临床病理因素无关;Smac/DIABLO蛋白表达与mRNA表达存在差异。Smac/DIABLO在肠型胃癌(84.38%)与弥漫型胃癌(60.65%)中的表达有一定差异(P<0.05)。结论:Livin与Smac/DIABLO在不同阶段和不同病理类型胃癌中的表达及相关性存在差异,Livin基因高表达与胃癌的发生及分化转移密切相关。Background and Objective. The proapoptotic gene Smac/DIABLO interacts with inhibitor of apoptosis protein Livin, which may contribute to regulating the impetus and progression of canceration. This study was to investigate the expression of Livin and Smac/DIABLO, and their correlations to the clinicopathological features in gastric adenocarcinomas. Methods. The expression levels of Livin and Smac/DIABLO mRNA in 75 gastric carcinoma specimens, 20 normal gastric tissues and 20 para-cancerous tissues were detected using the SYBR green real-time fluorescent quantitative reverse transcriptase PCR. The protein expression and location of Livin and Smac/DIABLO were measured by western blot and immunohistochemistry （SP method）. Results. The expression of Livin mRNA was significantly upregulated in gastric carcinomas （6.374±4.759） compared with the control.The expression of Livin was higher in poorly differentiated gastric carcinomas and carcinomas with lymph node metastases than in highly differentiated gastric carcinomas and carcinomas without lymph node metastases. （X^2=9.60, P〈0.01; X^2=5.51, P〈0.05）. The expression of Livin mRNA had no correlation to tumor site, and the invasion depth and TNM stage （P〉0.05）. In contrast, no expression of Livin was detected in normal and para-cancerous gastric tissues. The protein expression of Livin was consistent with that of its mRNA expression. The expression level of Smac/DIABLO mRNA was lower in gastric carcinomas （0.731±0.420） than in normal gastric tissues （1.104±0,276） and para-cancerous tissues （1.061±0.737）, but there was no significant difference （P〉0.05）. The expression of Smac/DIABLO mRNA was not correlated to clinicopathological characters of gastric adenocarcinoma.The protein expression of Smac/DIABLO was not completely consistent with that of its mRNA expression. The expression of Smac/DIABLO was different between intestinal type gastric carcinoma （84.38%） and diffuse type gastric carcinoma （60.65%） （P〈0.05）. Con

关 键 词：凋亡抑制蛋白 线粒体促凋亡蛋白 胃肿瘤 实时荧光定量 Western BLOT 免疫组化 

分 类 号：R735.2[医药卫生—肿瘤]