不同浓度绿茶活性成分协同作用抑制血管内皮细胞生长并诱导凋亡的效果分析  

Synergistic Effect of active Components of Green Tea on Angiogenesis

作　　者：何育玲[1] 朱宝和[2] 王敬芳[1] 

机构地区：[1]深圳市宝安区疾病预防控制中心,广东深圳518101 [2]南方医科大学附属宝安人民医院普外科,广东深圳518101

出　　处：《医学信息》2009年第6期907-909,共3页Journal of Medical Information

摘　　要：目的探讨不同浓度绿茶活性成分协同作用抑制血管内皮细胞生长并诱导凋亡的效果分析。方法采用不同浓度的绿茶四种主要活性成分(EGCG、ECG、EGC和EC)单独或联合作用于人脐静脉内皮细胞,检测血管内皮细胞的生长和凋亡。结果EGCG、ECG、EGC和EC都可以剂量依赖性地抑制血管内皮细胞生长和诱导其凋亡;其中,EGCG作用最强,小剂量的EGC、ECG和EC与EGCG联合应用可以显著增强EGCG抑制血管内皮细胞生长和诱导凋亡效应。结论不同浓度的绿茶主要活性成分都具有抑制血管内皮细胞生长和诱导凋亡作用,同时能够协同作用抑制血管生成。Objective To investigate the synergistic effect of active components of green tea on angiogenesis. Methods Human umbilical vein endothelial cells were treated with active components of green tea alone or united. The growth of vascular endothelial cells was studied by MTr assay, and the apoptosis by Annexin V-PI staining and flow cytometry. Results Active components of green tea inhibited the growth and induced the apoptosis of human umbilical vein endothelial cells. Jointed action of low dosage of ECG, EGC or EC with EGCG significantly enhanced the anti-angiogenesis effect of EGCG. Conclusions Active components of green tea synergistically inhibited the growth and induced the apoptosis of vascular endothelial cells.

关 键 词：绿茶 血管生成 增殖 凋亡 

分 类 号：R979.1[医药卫生—药品] R282.71[医药卫生—药学]