siRNA干扰c—FLIP表达治疗胆囊癌的实验研究  

Interference of c-FLIP by siRNA in treating gallbladder cancer

作　　者：宗华杰[1] 殷保兵[1] 陈进宏[1] 马保金[1] 蔡端[1] 何祥火[2] 

机构地区：[1]复旦大学附属华山医院普外科,上海200040 [2]上海交通大学肿瘤研究所,200032

出　　处：《中华肝胆外科杂志》2009年第5期374-378,共5页Chinese Journal of Hepatobiliary Surgery

摘　　要：目的检测c—FLIP在胆囊癌组织中的表达，探讨siRNA（small interfering RNA）干扰胆囊癌细胞c-FLIP表达后对细胞生长的影响。方法用免疫组化法检测10例正常胆囊组织，10例胆囊腺瘤和35例胆囊癌组织中c-FLIP的表达；siRNA干扰c—FLIP表达后，通过生长曲线测定、倒置相差显微镜检查和流式细胞仪检测细胞生长状况。结果在胆囊癌组织中，c—FLIP的表达明显高于在胆囊腺瘤（P〈O．05）和正常胆囊组织中的表达（P〈0．05）；siRNA干扰c—FLIP表达后，胆囊癌细胞株SGC-996的生长明显受到抑制，倒置相差显微镜检查发现，干扰后细胞凋亡细胞比例明显增加，流式细胞仪的检查发现干扰后细胞凋亡比例与对照组相比明显上升（P〈0．05）。结论siRNA干扰c-FLIP表达的靶向治疗有可能成为治疗胆囊癌的新的方法。Objective To investigate the expression of c-FLIP in gallbladder cancer and explore the effect of siRNA （small interfering RNA） to interfere expression of c-FLIP on gallbladder cancer cell line. Methods The expression of c-FLIP was detected by immunohistoehemical method in 35 cases of gallbladder cancer compared with 10 cases of normal gallbladder tissue and 10 cases of gallbladder adenoma. After being interfered by RNAi of e-FLIP, cell viability was measured by CCK-8 method, morphological changes observed through phase-microscopy and apoptosis detected by flow cytometry. Results The positive expression of c-FLIP in gallbladder cancer tissues was significantly higher than that in normal gallbladder tissues （P〈0.05） and gallbladder adenoma tissues （P〈0.05）. After being interfered by RNAi of c FLIP, cell growth of gallbladder cancer cell line SGC-996 was markedly inhibited. Either in phase-microscopy or in flow cytometry, the proportion of apoptosis in samples treated with RNAi of c-FliP for 24 hours was notablely increased as compared with those untreated samples（P〈0.05）. Conclusion Targeted therapy of RNAi of c-FLIP might be a promising method to treat gallbladder cancer.

关 键 词：胆囊肿瘤 C-FLIP 细胞凋亡 免疫组织化学 SIRNA 

分 类 号：R686[医药卫生—骨科学]