弯齿琵甲防御分泌物的化学成分测定及抗菌活性分析  

Chemical composition and antibiotic activity of the defensive secretion of Blaps femoralis

作　　者：李伟[1] 任国栋[1] 柳峰松[1] 

机构地区：[1]河北大学生命科学学院,保定071002

出　　处：《昆虫知识》2009年第3期424-427,F0003,共5页Entomological Knowledge

基　　金：国家自然基金重点项目(30130040);河北省无脊椎系统学与应用实验室资助项目(ISA003)

摘　　要：弯齿琵甲Blaps femoralis Fischervon Waldheim是中国北方的优势土壤昆虫,其防御腺分泌物用于御敌和引起本种群体的集结,体外具有一定毒性,民间将该虫作为药用昆虫资源利用年代久远。由该虫防御腺体获得分泌物并经气相色谱-质谱联用技术(GC-MS)分析,共计获得14种小分子化合物,其中以2-甲基苯醌、1-十三碳烯和2,4-二甲基-3-呋喃乙酮含量较高,苯醌、苯二酚、3,5-二羟基甲苯和十二烷醛等小分子化合物次之。研究证实该分泌物具有较明显的抑菌能力。研究结果为今后进一步深入开展弯齿琵甲的防御腺化学分泌物天然产物的研究积累了工作。Blaps femoralis Fischer yon Waldbeim is a dominant soil beetle in Northern China. Its defensive secretion is normally used for defense and aggregation in the daily activities. The secretion is toxic and has been used in natural medicines for many years. Through combined gas chromatography-mass spectroscopy technique （GC-MS）, it showed that the defensive secretion contained 14 kinds of compound. Three kinds of compound with higher amount were identified as 2- methyl- p- benzoquinone, 1-tridecene, and 1-（2, 4- dimethylfuran- 3 - yl）- ethanone. Some of remaining compounds with relative amount were identified as p-benzoquinone, hydroquinone, 3, 5-dihydroxytoluene, and dodecanal. We also found that the defensive secretion had obvious antibacterial activity. This work established foundation for future intensive study of the natural products from the defensive gland of B. femoralis.

关 键 词：弯齿琵甲 防御分泌物 化学成分 气相色谱-质谱联用技术(GC-MS) 抑菌 

分 类 号：Q96[生物学—昆虫学]