鸡TLR4基因表达水平与沙门氏菌感染关系的研究  

Study on the Relationship between Chicken Toll Like Receptor 4 Expression Level and Salmonella Infection

作　　者：李鹏[1,2] 夏平安[1] 宋咏梅[2] 赵桂苹[2] 

机构地区：[1]河南农业大学牧医工程学院,郑州450002 [2]中国农业科学院北京畜牧兽医研究所,北京100193

出　　处：《中国畜牧兽医》2009年第5期72-74,共3页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金(30871776)

摘　　要：为了研究鸡TLR4(toll like receptor 4)基因表达在调控鸡沙门氏菌感染中的作用,80只3日龄SPF鸡按随机分为2组,处理组经口注射鸡白痢沙门氏菌悬液108CFU/mL 0.50 mL,对照组注射同剂量生理盐水。每个处理组分别在感染后1、3、7和14 d随机取8只鸡处死,迅速取脾脏样品用于RNA提取。采用SYBR GreenⅠ染料实时荧光定量PCR(QPCR),比较处理组和对照组TLR4基因mRNA相对表达量的变化。结果显示,在4个时间点,与对照组相比,处理组TLR4基因高表达,差异显著(P<0.05);尤其在感染后3和7 d,差异达到极显著水平(P<0.01)。结果表明,鸡沙门氏菌感染后,TLR4基因mRNA表达上调,TLR4基因在沙门氏菌感染中发挥重要作用。In order to study the role of chicken toll like receptor 4 playing in Salmonella infection, eighty three-day-old specific-pathogen-free chickens were randomly divided into 2 groups. The treatment group chickens were orally inoculated with 0. 5 mL of the bacterial suspension containing 108 CFU/mL Salmonella Serovar Pullorum. The control groups were inoculated with 0.5 mL saline. Eight chickens of each group were randomly chosen and killed at day 1, 3, 7 and 14 post infection. Spleen was sampled for RNA extraction. SYBR Green I quantitative real-time PCR method was applied to compare the relative expression quantity of chicken TLR4 gene. The following results were obtained： TLR4 gene expression levels were significantly higher in chickens from treatment group than those in the control group at 4 time points （P〈0.05）, especially at day 3 and 7 post infection （P〈0.01）. These results indicated that the expression of chicken TLR4 gene was up-regulated during Salmonella infection.

关 键 词：鸡 TLR4基因 沙门氏菌 荧光定量PCR 

分 类 号：Q781[生物学—分子生物学]