猪垂体特异性转录因子1基因cDNA的克隆及序列分析  

Cloning and Sequence Analysis of cDNA of Swine POU1F1 Gene

作　　者：孙丽亚[1] 宋成义[1] 高波[1] 赵芹[1] 王宵燕[1] 周智慧[1] 王升智[1] 周辉云[1] 李碧春[1] 

机构地区：[1]扬州大学动物科学与技术学院,扬州225009

出　　处：《中国畜牧兽医》2009年第5期92-94,共3页China Animal Husbandry & Veterinary Medicine

基　　金：江苏省自然科学基金(用小基因模型研究家猪POU1F1基因突变对mRNA可变剪接的影响;BK2006068)

摘　　要：本试验根据公开发表的猪POU1F1基因序列设计1对引物,提取猪垂体总RNA,通过RT-PCR方法扩增出POU1F1基因cDNA全序列,扩增产物用琼脂糖凝胶检测为预期的876 bp特异性条带。将扩增产物克隆入PTZ57R/T载体进行序列测定。经DNAStar软件分析序列与已发表序列同源性为99.7%,克隆获得的序列为进一步对猪POU1F1基因不同拼接形式功能性研究奠定了基础。A pair of primers was designed to clone the POU1F1 cDNA according to published swine POU1F1 mRNA sequence. The total RNA was extracted from swine pituitary and the full length cDNA of swine POU1F1 was amplified by reverse transcription-polymerase chain reaction （RT-PCR）. The PCR product with expected size of 876 bp was inserted into PTZ57R/T vector and sequenced. Finally the result sequence was analyzed by DNAStar and it demonstrated 99.7% homology with published sequence in GenBank. The cloned cDNA will be used for further function research of alternative splicing of swine POU1F1 Gene .

关 键 词：POU1F1 CDNA 克隆 序列分析 

分 类 号：Q785[生物学—分子生物学]