中国河南两株庚型肝炎病毒(HGV)NS5区部分cDNA的克隆与序列分析  

CLONING AND SEQUENCE ANALYSIS OF HEPATITIS G VIRUS IN PARTIAL NS5 REGION ISOLATED FROM TWO BLOOD DONORS IN HENAN PROVINCE

作　　者：谭文杰[1,2] 陈刚[1,2] 夏宁邵[1,2] 黄鹤丛 郁苗季 詹美云[1,2] 

机构地区：[1]中国预防医学科学院病毒学研究所 [2]厦门大学生物系

出　　处：《病毒学报》1998年第2期114-120,共7页Chinese Journal of Virology

基　　金：卫生部"九五"攻关课题;国家863课题

摘　　要：通过逆转录－聚合酶链反应从我国河南省２例重叠感染ＨＣＶ或ＨＢＶ／ＨＤＶ的献血员中，分离到ＨＧＶＮＳ５区的部分ｃＤＮＡ，对其进行序列分析比较，结果表明：河南株ＨＧＶＮＳ５区核苷酸与两株中国ＨＧＶ株的同源性（＞９１．９％）高于国外代表株（８８．５％－９０．６％），但由核苷酸推导的氨基酸的同源性都无明显的地区性区别。ＨＧＶＮＳ５区氨基酸序列较保守（同源性＞９１％），缺乏明显高变区。中国４株ＨＧＶ在７３８４位发生了由Ｃ→Ｔ的变异，从而导致一个共同的保守位点的产生（由Ｆ→Ａ）。重叠感染其他肝炎病毒时。Non-structural region 5(NS5)gene fragments were isolated from two blood donors in Henan Province who were co-infected with hepatitis C or B/D viruses.These fragments were obtained by using reverse transcription and polymerase chain reaction(RT-PCR),and these overlapping fragments were cloned into pGEM-T vector.Sequences were determined and then analyzed by computer.The results indicated as follows:the nucleotide identity of HGV NS5 cDNA among the Henan isolates and the other Chinese isolates(HGVCN,HGVC964)were higher(>91.9%)than that among Henan and foreign country(Africa,American,Japan,Europe)isolates(88.5%-90.6%);but the amino acid homology didn’t show any significant difference among them.The NS5 fragments of HGV strains compared were very conserve(above 91.9% identical to each other)in amino acid level and didn’t exist distinct hypervariable region.Four Chinese isolates of HGV consisted a common variation(C→T)in 7384 nucleotide (the position number according to U44402)and this led to a unique amino acid variation(F→A).It can be suggested that the HGV fragments isolated from Henan area didn’t exist characteristic change when they were co-infected with other hepatitis viruses.

关 键 词：庚型肝炎病毒 非结构基因 序列分析 

分 类 号：R373.21[医药卫生—病原生物学]