巢式PCR-RFLP检测大便K-ras基因第12位密码子点突变  

Detection of Point Mutation at Codon 12 of K-ras Gene in Feces by Nested PCR-RFLP

作　　者：肖尚喜[1] 徐阿曼[1] 王道斌[1] 吴琳[1] 刘华平[1] 郑冰[1] 

机构地区：[1]安徽医科大学第一附属医院,合肥230022

出　　处：《中国生物制品学杂志》1998年第2期103-106,共4页Chinese Journal of Biologicals

基　　金：安徽省卫生厅医学科研基金!94-04

摘　　要：用巢式PCR－RFLP分析技术，扩增大便中K－ras基因第12位密码子所处的一段外显子，根据BstNI内切酶的多态性，分析K－ras基因第12位密码子是否突变。结果在22例结肠直肠癌患者的术后石蜡包埋组织中，检出10例（45.5％）K－ras突变，该10例患者中，有8例（36．4％）患者的术前大便中检出K－ras基因突变。这种方法快速、简便、特异、敏感，且可避免大便中多种物质对PCR的干扰。对可疑人群具有实际应用价值。A fragment of extron at codon 12 of K-ras gene in feces was amplified bynested PCR-RFLP and degisted with restriction enzpoe Bst NI.K-ras gene mutations weredetected out in the pathological tissues of 10 of 22 patients with co1onic and rectal cancers after operation.However,the mutations were detected out in the feces specimens from 8 of the22 patients before operation.The method was rapid,simple,specific,sensitive and not interfered with other materials in feces.It could be app1ied for the screening of doubtful patientswith colonic and rectal cancers.

关 键 词：聚合酶链反应 RAS基因突变 大肠肿瘤 

分 类 号：R734.340.4[医药卫生—肿瘤]