多柔比星葡聚糖纳米粒对卵巢癌细胞SKOV3的杀伤作用及研究  

Preparation and anti-oophoroma effect of doxorubicin encapsulated in amphiphilic polysaccharide nanoparticles

作　　者：何肇晴[1] 曹翠琴[1] 李霞[1] 陈丹[1] 程杰[1] 程冬英[1] 李婧[1] 汪锐[1] 龚皓[1] 杨颖[1] 

机构地区：[1]武汉第一医院肿瘤科,武汉430022

出　　处：《癌症进展》2009年第3期335-338,334,共5页Oncology Progress

基　　金：武汉市科学技术计划项目(项目编号:Z20036002005)

摘　　要：目的制备载多柔比星葡聚糖纳米粒(DOX/DEX-PLA),观察其药物缓释规律,探讨其对卵巢癌细胞的杀伤作用。方法以双乳化剂蒸发法制备DOX/DEX-PLA载药纳米粒,透射电镜观察纳米粒形态,分光光度法计算载药率,体外药物释放实验考察纳米粒对DOX的缓释作用。MTT法观察对卵巢癌细胞的体外杀伤作用,动物实验观察其体内抑瘤效应。结果DOX/DEX-g-PLA纳米微粒呈球形,粒径约83 nm,药物包封率约67.1%。体外释放实验提示,纳米制剂中约50%的药物持续缓慢释放,可持续达7天;体内抑瘤实验显示,纳米缓释制剂间隔给药疗效优于未包载药物每日给药的疗效。结论DOX/DEX-PLA纳米粒可有效抑制卵巢癌细胞的生长。To investigate the preparation of DOX/DEX - PLA nanoparticles and investigated their effect of killing oophoroma cells in vitro and in vivo. The DOX/DEX - PLA nanoparticles were prepared by the method of emulsification ＆ evaporation of organic solvent. Its morphology was observed by transmission electron microscopy, and the encapsulating efficiency of DOX was determined by ultraviolet spectrophotometry. The inhibitory effect against oophoroma cells in vitro was measured by MTT assay. Results The DOX/DEX - PLA nanoparticles were of round or elliptical shape. The diameter of the nanoparticles was about 83 nm, and the entrapment efficiency was about 67.1%. The releasing test in vitro manifested that over 50 percent of DOX encapsulated in DOX/DEX - PLA nanoparticles were sustained released for about 7 days. As a result, the Anti - oophoroma Effect in vivo indicated that the efficacy of control -releasing agent by interval drug delivery has advantages over one of the unenveloped drug by per diem drug delivery. Conlusions DOX/DEX -PLA phoroma cells. nanoparticles can effectively inhibit the growth of oophoroma cells.

关 键 词：葡聚糖 纳米 多柔比星 卵巢癌 抗肿瘤 

分 类 号：R737.31[医药卫生—肿瘤]