野牦牛mtDNA Cytb基因全序列测定及系统进化关系  

Sequence Analysis and Evolution Relatinship of Cytb Gene in Bovidae

作　　者：杨万远[1] 陈雪梅[1] 钟金城[1] 陈生梅[2] 郭承恩[1] 孟丽[1] 武甫德[3] 

机构地区：[1]西南民族大学动物遗传育种学国家民委-教育部共建重点实验室,成都610041 [2]青海省畜牧兽医科学院 [3]青海省大通种牛场

出　　处：《中国草食动物》2009年第3期8-13,共6页China Herbivores

摘　　要：为从分子水平探究牦牛的分类地位和遗传多样性,试验测定了野牦牛细胞色素b基因全序列,并以绵羊为外群,构建野牦牛、家牦牛、大额牛、普通牛、瘤牛、水牛、非洲野牛、欧洲野牛、美洲野牛、非洲水牛等牛亚科种间系统进化树。结果表明:野牦牛细胞色素b基因全序列长1 140bp,序列间共有13个SNP多态位点,核苷酸变异类型包括转换和颠换,无插入和缺失,表明野牦牛具有较丰富的遗传多样性。研究结果支持将牦牛划分为牛亚科中一个独立属(即牦牛属)的观点。In order to explore the classification status and genetic diversity of wild yak at the molecular level, the cytb gene sequences of yak were determined,taking the sheep as the outside group to construct phylogenetic trees including wild yak,domestic yak,cattle,zebu,water buffalo, African buffalo, the European bison, American bison, and other Subfamily Bovidae species. The results showed that the length of wild yak cytb gene sequence was all 1 140bp and there were more than 13 nucleotide variations exsisted among sequences which including nucleotide conversion and transversiohs, no insertion and deletion that indicated the genetic diversity was rich in wild yak. The results also supportted taking the yak as an independent genus （that is yak genus） point of view.

关 键 词：野牦牛 线粒体DNA 细胞色素B 起源 进化 

分 类 号：S884.52[农业科学—特种经济动物饲养] S852.659.6[农业科学—畜牧兽医]