没食子酸抑制白念珠菌生物膜作用的研究  

In vitro activity of gallic acid against Candida albicans biofilms

作　　者：汪长中[1] 程惠娟[1] 官妍[1] 王艳[1] 云云[1] 

机构地区：[1]安徽中医学院中西医结合临床学院,安徽合肥230038

出　　处：《中国中药杂志》2009年第9期1137-1140,共4页China Journal of Chinese Materia Medica

基　　金：安徽省高校自然科学研究项目(KJ2008B223);安徽省自然科学基金项目(07021003)

摘　　要：目的:研究没食子酸对体外白念珠菌生物膜的影响。方法:采用XTT减低法评价没食子酸对白念珠菌的生物膜及黏附性的影响;镜下观察没食子酸对白念珠菌生物膜的形态学影响;细胞毒试验检测该药的毒副作用。结果:没食子酸抑制白念珠菌生物膜最低药物浓度SMIC50,SMIC80分别是500,1 000 mg.L-1;100,1 000 mg.L-1的没食子酸对白念珠菌的早期黏附及菌丝生长有抑制作用;没食子酸对人细胞毒性较弱。结论:没食子酸对体外白念珠菌生物膜有较强的抑制作用。Objective： To investigate the effects of gallic acid against Candida albicans biofilms in vitro. Method： XTT reduction assay was performed to determine the effect of gallic acid on C. albicans biofilms and its adherence, and microscopic examination was conducted to assess the effect of gallic acid on morphogenesis of C. albicans biofilms ; and cytotoxic assay was used to measure the adverse effects of gallic acid. Result： SMIC50, SMIC80 of gallic acid against C. albicans biofilms were 500, 1 000 mg.L^-1 , respectively; 100 mg.L^-1 and 1 000 mg.L^-1 of gallic acid could inhibit the initial adherence and filamentous growth, and the agent showed poor cytotoxic activity. Conclusion： gallic acid displayed potent activity against C. albicans biofihn.

关 键 词：白念珠菌 生物膜 没食子酸 

分 类 号：R285.5[医药卫生—中药学]