谷氨酰胺高效酶法转化条件研究  

The study of Efficient Enzymatic Conversion of Glutamine

作　　者：周丛 李宗军[2] 

机构地区：[1]国家农副产品质量监督检验中心 [2]湖南农业大学食品科技学院,中国湖南长沙410007

出　　处：《氨基酸和生物资源》2009年第2期46-49,66,共5页Amino Acids & Biotic Resources

摘　　要：谷氨酰胺合成酶是生物体氮代谢的中心酶之一,在消耗ATP的情况下,谷氨酰胺合成酶催化由谷氨酸和NH4+向谷氨酰胺的转化,Toch ikura提出了将酵母发酵与纯化酶结合生产谷氨酰胺(G ln)的方法,本实验通过建立酶法合成L-G ln与酵母酒精发酵的能量偶联体系,研究了在此偶联体系中各因素对谷氨酰胺酶转化效率的影响,为工业上利用酶法生产G ln提供理论依据。Glutamine synthetase is very important enzyme for human. By ATP consumption, glutamine synthetase catalyzes glutamic acid and NH4 ^＋ to conversion of glutamine, Tochikura have made the yeast fermentation combined with the net production of glutamine. We established energy coupling system of enzymatic synthesis of L - Gin and the yeast fermentation of alcohol, and different factors influenced the glutamine conversion were studied.

关 键 词：L-谷氨酰胺 生物酶法 转化条件 

分 类 号：Q503[生物学—生物化学]