中华猕猴桃多糖对小鼠皮下移植瘤B16抑制的机制研究  

Antitumor effect and its mechanism of actinidia Chinensis planch polysaccharide on B16-bearing mice

作　　者：石森林[1] 潘国凤[2] 张晓东[3] 李昌煜[1] 葛卫红[1] 

机构地区：[1]浙江中医药大学药学院,杭州310053 [2]中国中医科学院,北京100700 [3]国家食品药品监督管理局药品审评中心,北京100038

出　　处：《中华中医药杂志》2009年第6期777-779,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：浙江省自然科学基金项目(No.300431)

摘　　要：目的:考察中华猕猴桃多糖(ACPS)对黑色素瘤B16小鼠皮下移植瘤有无抑制作用与作用强度,并探讨其抗肿瘤作用机制。方法:采用B16小鼠皮下移植瘤法建立实验动物模型,接种瘤株24h后尾静脉注射给药,隔天1次,共7次,实验结束测算瘤重、抑瘤率及脾脏和胸腺的质量与相应指数,移植瘤制备单细胞悬液,采用流式分析法了解给药后细胞周期分布改变,并制作电镜标本从形态学观察其细胞凋亡情况。结果:ACPS能抑制小鼠皮下移植瘤B16的生长,高、中两个剂量组抑瘤作用显著,抑瘤率分别为48.68%、40.90%;各剂量组均能显著提高脾脏指数,其中高剂量组与空白对照组相比其提高具有非常显著的差异(P<0.01);流式细胞分析结果表明ACPS实验组G1期与S期的细胞比例增加,增殖指数降低;透射电镜观察ACPS实验组肿瘤细胞出现典型的凋亡形态学变化,可以找到典型的凋亡小体。结论:ACPS在体内对B16黑色素瘤有明显的抑制作用,其机制可能与调节机体免疫及诱导肿瘤细胞的凋亡相关。Objective： To investigate the antitumor effect of Actinidia Chinensis Planch polysaccharide （ACPS） in B 16- bearing C57BL/6 mice and approach its mechanism. Methods： The C57BL/6 mice model was established by B16 subcutaneous inoculation, giving the polysaccharides from vena caudalis injection, and flow cytometer （FCM） was used to detect the distribution of tumor-cell cycle, and electron microscope was used to survey morphologic transformation about apoptosis. Results： ACPS can inhibit the growth of B16, the high, middle dose groups obviously restrained the tumor with the rate of 48.67%, 40.90%and the control group 24.13%, and classical apoptosis corpuscles had been found through electron microscope in ACPS groups. Compared with control group, the ACPS promoted the spleen-index of B16-bearing mice and cut down the proliferation index, and the G1/S phase was at growth-inhibitory concentrations judged by the distributing analysis on cell-cycle. Conclusion： ACPS had obvious effect of restraining B16 and promoting the spleen index of tumor-beating mice, which maybe due to its function of regulating immunization and the distributing of cell-cycle.

关 键 词：中华猕猴桃多糖 抗肿瘤 作用机制 免疫 凋亡 

分 类 号：R285.5[医药卫生—中药学]