家蝇抗菌物质诱导及高温处理后的抑菌活性  

Effect of high temperature on antibacterial activity of antibacterial substances induced in the house fly (Musca domestica) by pricking

作　　者：许兵红[1] 董卫华[1] 陈萍[1] 曾莉萍[2] 

机构地区：[1]新乡医学院寄生虫学教研室,河南新乡453003 [2]成都医学院生物化学实验室

出　　处：《中国媒介生物学及控制杂志》2009年第3期201-205,共5页Chinese Journal of Vector Biology and Control

基　　金：河南省教育厅自然科学基础研究项目(2007310010)

摘　　要：目的探讨家蝇幼虫经针刺诱导产生的抗菌物质经不同温度处理不同时间后的抑菌活性。方法室内饲养的家蝇3龄幼虫,经针刺诱导48h后提取血淋巴,100℃处理10s和30s及1、3、5min,80℃和60℃处理30s、1、3、5、10min后,采用磷酸盐缓冲液制备离心上清样品,分别观察记录其对溶壁微球菌和金黄色葡萄球菌的抑菌效果。结果经100、80℃处理后,对溶壁微球菌的抑菌环随处理时间的延长而环直径减小,60℃处理不同时间对溶壁微球菌的抑菌环大小无明显影响。100℃处理1min后以及80℃处理3min后对金黄色葡萄球菌无明显的抑菌环产生,60℃处理不同时间对金黄色葡萄球菌的抑菌环直径无明显影响。结论家蝇幼虫经针刺诱导产生的抗菌物质,其抑菌活性对高温有一定的稳定性,80～100℃加热处理,抑菌活性随处理时间延长而减弱,60℃高温处理10s至10min对其抑菌活性无明显影响。Objective To investigate the effect of high temperature on antibacterial activity of antibacterial substances induced in the larvae of house fly by pricking. Methods The induced hemolymph was bathed at 100 ℃ for 10 sec, 30 see, 1 min, 3 min, 5 min, 80 ℃ and 60 ℃ for 30 sec, 1 min, 3min, 5 min, 10 rain, then solved in the buffer solution of Na2HPO4- KH2PO4（1/15 mol/L, pH 6.5） and centrifuged, the supernatant was used to test the bacteriostasic efficiency to Micrococcus lysodeikticus and Staphylococcus aureus respectively. Results Diameter of antibacterial ring against M.lysodeikticus decreased along with the increase of bath time at both 100 ℃ and 80 ℃. No antibacterial ring against S.aureus was found after bathed at 100 ℃ for 1 min or 80 ℃ for 3 min. Diameters of antibacterial ring were similar after bathed at 60 ℃for different time against both M.lysodeikticus and S.aureus. Conclusion It suggested that the antibacterial activity of antibacterial substance induced by pricking in the house flv was stable against high temperature in a certain extent. Antibacterial activity weakened along with the increase of bath time at 100 ℃ and 80 ℃, and no obvious change after bathed at 60 ℃ for 10 s- 10 min.

关 键 词：家蝇 抗菌物质 诱导 高温 

分 类 号：R384.2[医药卫生—医学寄生虫学]