Staurosporine诱导心肌细胞氯通道电流的电生理学特性  

Electrophysiological characteristics of chloride channel currents in cardiomyocytes induced by staurosporine

作　　者：刘艳[1] 张卫卫[1] 李源[1] 王晓明[1] 

机构地区：[1]第四军医大学西京医院老年病科,陕西西安710032

出　　处：《心脏杂志》2009年第3期292-295,共4页Chinese Heart Journal

基　　金：国家自然基金课题项目资助(30570758;30770847)

摘　　要：目的探讨staurosporine(STS)诱导乳鼠心肌细胞凋亡的早期,凋亡性容积减少(AVD)发生时,是否有氯通道电流的产生及其电生理学特性。方法分别采用低渗组的灌流液和含STS的等渗组灌流液处理原代培养的SD乳鼠心肌细胞,以膜片钳全细胞记录法记录电流。结果①以低渗灌流液处理的心肌细胞时,可记录到氯通道电流。该电流呈现类似容积敏感性氯通道电流(volume-sensitive chloride channel current,ICl,Vol)的电生理学特性:即外向整流性、高电位刺激下的时间依赖性失活及对氯通道阻断剂4,4′-异二硫氮氐2,2′-二磺酸(DIDS)的敏感性。②以含4μmol/L STS的等渗液灌流心肌细胞时,也可记录到类似低渗诱导产生的氯通道电流,具有ICl,Vol的电生理学特性,且用氯通道阻断剂500μmol/L DIDS后,在+40 mV、+60 mV、+80 mV及+100 mV时,能够明显电压依赖性地阻断该电流。结论首次应用STS在培养乳鼠心肌细胞中记录到类似容积敏感性氯通道电流。AIM To explore whether chloride channel currents are present when apoptotic volume decrease （AVD） happens during the early times of neonatal rat eardiomyocytes apoptosis induced by staurosporine （STS）, and to study the electrophysiological properties of chloride channel currents. METHODS Primary cultured neonatal rat cardiomyocytes were subjected to hypotonic and isotonic peffusion containing 4μ mol/L STS, respectively. We used whole-cell patch-clamp techniques to record chloride channel currents. RESULTS （1）Afler exposure of cardiac myocytes to hypotonic solution, we recorded chloride channel currents which resembled volume-sensitive chloride channel currents （Icl,vol） ： outward rectification, time dependent inactivation, sensitivity to 4,4＇-diisothiocya-natostilbene-2,2＇-dis- ulfonicaeid （DIDS）. （2）Afler exposure of cardiac myocytes to 4 μ mol/L STS isotonic solution, chloride channel currents showing characterizations of Icl,Vol were also recorded. The currents were voltage depend- ently blocked by 500 μmol/L DIDS at ＋40mV, ＋60mV, ＋80mV, ＋100mV. CONCLUSION It＇s the first time to record similar volume-sensitive outwardly rectifying Cl-currents by STS in primary cul- tured neonatal rat cardiomyoeytes.

关 键 词：心肌细胞 STAUROSPORINE 容积敏感性氯通道电流 全细胞记录 

分 类 号：R331.38[医药卫生—人体生理学]