益气活血复方芪丹通脉片对缺血/再灌注大鼠心肌中NF-κB表达的影响  

Effect of qidantongmai tablet on NF-κB expression in myocardium of rats with ischemia reperfusion injury

作　　者：解娟[1] 王宗仁[1] 王跃民[2] 李军昌[1] 黄烨[1] 

机构地区：[1]第四军医大学西京医院中医科,陕西西安710032 [2]第四军医大学基础部生理学教研室,陕西西安710032

出　　处：《心脏杂志》2009年第3期325-328,共4页Chinese Heart Journal

基　　金："十一.五"军队中医药重大临床攻关专项课题基金资助(2006191001)

摘　　要：目的探讨益气活血复方芪丹通脉片(QDTMT)对缺血/再灌注(I/R)大鼠心肌中核因子-κB(NF-κB)表达的影响。方法将SD大鼠48只随机分为6组(n=8),即假手术组、模型组、QDTMT低中高剂量组(0.36、1.08、3.24g/kg)及恬尔心组(5 mg/kg)。各组均用生理盐水配制的等体积药液灌胃7 d,每日1次。采用冠脉结扎的方法,造成大鼠心肌I/R模型后,采用Western blot和免疫组化染色法,检测各组大鼠心肌中NF-κB蛋白的表达。结果与假手术组比较,模型组NF-κB的表达显著增多(P<0.01)。与模型组比较,QDTMT各剂量组和恬尔心组NF-κB的表达显著减少(P<0.01)。结论QDTMT能抑制I/R大鼠心肌中NF-κB蛋白的表达,从而对心肌具有保护作用。AIM To investigate the effect of qidantongmai tablet（ QDTMT） on NF-kB in the rat model of ischemia reperfusion-mediated myocardial injury （RMMI）. METHODS Forty-eight male SD rats were randomly divided into six experimental groups： sham operation group, control group, low dose QDTMT （0.36 g/kg） group, medium dose QDTMT（1.08 g/kg） group, high dose QDTMT（3.24 g/kg） group and dihiazem hydroehloride（5 mg/kg） group. Different dosage of drug or saline at the same volume was administered to the animals once a day for seven days before operation. The model was established by ligating the coronary artery. Tissues in the reperfused region were sampled immediately and the protein levels of NF-KB were determined by Western bolt and immunohistochemical staining. RESULTS Com- pared with that in the sham operation group, the expression of NF-KB in the control group was obvious and the level of total NF-KB increased. Compared with that of control group, the expression of NF-KB was not obvious and the level of total NF-KB decreased in different QDTMT dosage groups as well as the diltiazem hydrochloride group. CONCLUSION QDTMT has some cardioprotective effect against RMMI in rats possibly by inhibiting the expression of NF-kB.

关 键 词：心肌缺血/再灌注 NF—KB表达 芪丹通脉片 SD大鼠 

分 类 号：R289.5[医药卫生—方剂学]