^(60)Coγ射线诱导的弥漫性皮质发育不良模型鼠脑组织病理特征观察  

Pathologic features of injury in ^(60)Co γ-radiation-induced diffuse cortical dysplasia in rats

作　　者：罗春阳[1] 马勋泰[2] 晏勇[2] 王学峰[2] 晏宁[2] 

机构地区：[1]重庆市中山医院神经内科,重庆400013 [2]重庆医科大学附属第一医院神经内科,重庆400016

出　　处：《重庆医科大学学报》2009年第6期657-660,共4页Journal of Chongqing Medical University

基　　金：国家自然科学基金资助项目(30570636);重庆市教委应用基础研究基金渝教科(2001)12-29;重庆市医学科技基金(01-1-013)

摘　　要：目的:建立γ-射线诱导的弥漫性皮质发育不良(Diffuse cortical dysplasia,DCD)大鼠模型,观察其脑的组织病理特征,探讨病理与致痫机制的关系。方法:用γ-射线诱导DCD大鼠模型。观察DCD鼠的行为和脑电图的变化,采用HE、Nissl、LFB和Timm’s硫化银组织化学方法染色及细胞凋亡-Hoechst染色法观察组织病理学特征。结果:DCD组少数自发性癫痫发作,大多表现活动增多、兴奋躁动、搔抓和"洗脸样活动"频繁。额叶皮质及海马EEG示频繁自发的棘波、尖波、棘慢综合波等发放。DCD组脑皮质、海马结构紊乱,细胞结构异常,双侧海马CA3区均有苔藓纤维发芽(P<0.05)。皮质和海马结节及周围有致密浓染的凋亡细胞核。结论:γ-射线诱导的皮质发育不良是模拟人类皮质发育不良、室周结节性异位、胼胝体缺失的理想动物模型。Objective：To establish neonatal γ-ray irradiated injury model of diffuse cortical dysplasias（DCD） in rats to observe the features of pathology, and discuss the mechamism of pathology and epileprogenesis in this model. Methods：γ-ray-induced model of DCD was established in rat. We observed the cerebral cortex change with naked eyes and under a light microscope,and assessed the hippocampal mossy fiber sprouting after HE staining, Nissl staining and Timm＇s histochemical method. The comparisons between every two groups were performed with Nemenyi test. Results：The rats had seizure by chance,but most of these rats displayed more active action,exiting and so on.EEG showed frequent spontaneous sharp waves,or spikes from the frontal or the hippocampal neocortical structures. Neonatal γ-ray irradiated injury could result in DCDs in cerebral cortex and mossy fiber sprouting in hippocampi CA3 area bilaterally （nonparametric Kruskal-Wallis H test and Nemenyi test,P〈0.05）. Conclusion：Neonatal γ-ray irradiated injury could result in an ideal animal model simulating human cortical dysplasia, periventricular nodular heterotopia, and loss of lamination.

关 键 词：癫痫 皮质发育障碍 动物模型 海马 苔藓纤维 病理学 

分 类 号：R742.8[医药卫生—神经病学与精神病学]