化疗药物联合TRAIL对鼻咽癌细胞株增殖抑制的实验研究  

Effects of Trail combined with chenotherapeutics on proliferation and apoptosis of HNE-1 cell line

作　　者：余晓燕[1] 邓碧[1] 沈娜[1] 叶琳[1] 张潜英[1] 陈鸿雁[1] 

机构地区：[1]重庆医科大学附属第一医院耳鼻咽喉科,重庆400016

出　　处：《重庆医科大学学报》2009年第6期709-711,共3页Journal of Chongqing Medical University

基　　金：重庆市卫生局资助(项目编号:07-1-021)

摘　　要：目的:探讨肿瘤坏死因子相关凋亡诱导配体(TNF related apoptosis indncing ligmard,TRAIL)联合化疗药物顺铂(Cisplatin,DDP)、5氟尿嘧啶(5-Fluorouracil,5-FU)作用于鼻咽癌HNE-1细胞株后对其细胞毒作用及凋亡影响的研究。方法:MTT法检测其增殖抑制率,FCM法检测细胞凋亡率。结果:TRAIL与DDP、5-FU联合作用于HNE-1细胞株时,其抑制率、凋亡率高于单独作用时的抑制率和凋亡率。结论:TRAIL与5-FU、DDP联合作用有协同抑制HNE-1细胞株增殖、诱导HNE-1细胞株凋亡的作用。Objective：To observe the effects of different concentrations of TRAIL and chemotherapeutic drugs（5-FU, DDP） on proliferation and apoptosis of HNE-1 cell lines ,both singlely and jointly. Methods：MTT was used to detect the inhibition rate. FCM was used to detect the apoptosis rate of cell.Results：The inhibition rate and apoptosis rate of combination use of TRAIL and 5-FU, DDP were significantly increased,compared with those of single use of TRAIL.Conclusion：The inhibition of proliferation and apoptosis of HNE-1 cell line can be induced by TRAIL, and the effects were higher by TRAIL combined with 5-FU and DDP.

关 键 词：TRAIL DDP 5-FU HNE-1细胞株 

分 类 号：R739.6[医药卫生—肿瘤]