HERG K+ channels expression in gastric cancers and analysis of its regulation in tumor cell proliferation and apoptosis  

作　　者：Qing Lu Huiyu Li Xiaoming Lu Guobin Wang 

机构地区：[1]Department of Gastrointestinal Surgery, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China [2]Center for Stem cell Research and Application, Institute of Hematology, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China

出　　处：《Journal of Nanjing Medical University》2009年第3期157-162,共6页南京医科大学学报（英文版）

基　　金：supported by a grant from the Natural Science Foundation of China(30772128)

摘　　要：Objective： To investigate the expression of hergl gene in tumor tissues from gastric carcinomas and gastric carcinoma cell lines, and study the relationship between HERG K＋ channel expressions and tumor cell proliferation and apoptosis. Methods： RT-PCR and PCR assays were used to detect the expression of hergl gene in 64 gastric carcinomas and the gastric cancer cell line SGC-7901. Blocking the HERG K＋ channels was used to evaluate their effects on tumor cell proliferation and apoptosis. Results：The statistically significant expression of hergl gene was detected in all the gastric cancers and SGC-7901 cells, but not in normal tissues. The HERG K＋ channel blocker, E-4031, increased the cell population in G0/G1（P 〈 0.05） and the number of apoptotic tumor cells（P 〈 0.05）. Conclusion： HERG K＋ channels were expressed in all gastric carcinomas tested and these channels appear to modulate tumor cell proliferation and apoptosis.Objective： To investigate the expression of hergl gene in tumor tissues from gastric carcinomas and gastric carcinoma cell lines, and study the relationship between HERG K＋ channel expressions and tumor cell proliferation and apoptosis. Methods： RT-PCR and PCR assays were used to detect the expression of hergl gene in 64 gastric carcinomas and the gastric cancer cell line SGC-7901. Blocking the HERG K＋ channels was used to evaluate their effects on tumor cell proliferation and apoptosis. Results：The statistically significant expression of hergl gene was detected in all the gastric cancers and SGC-7901 cells, but not in normal tissues. The HERG K＋ channel blocker, E-4031, increased the cell population in G0/G1（P 〈 0.05） and the number of apoptotic tumor cells（P 〈 0.05）. Conclusion： HERG K＋ channels were expressed in all gastric carcinomas tested and these channels appear to modulate tumor cell proliferation and apoptosis.

关 键 词：gastric carcinoma HERG K＋ channel herg 1 gene PROLIFERATION APOPTOSIS 

分 类 号：R972[医药卫生—药品] R73-36[医药卫生—药学]