亚硒酸钠和维甲酸联合对HL-60细胞c-myc和c-fos基因表达的影响  

Combined effects of sodium selenite and all-trans retioic acid on c-myc and c-fos oncogenes expression of human pronyelocytic leukemia cells(HI-60)

作　　者：刘新光[1] 于树玉[2] 

机构地区：[1]广东医学院医用生化研究所,湛江524023 [2]中国医学科学院肿瘤研究所生化室

出　　处：《肿瘤防治研究》1998年第3期173-177,共5页Cancer Research on Prevention and Treatment

摘　　要：以HL－60细胞为实验对象，利用Slot－blot技术，观察了亚硒酸钠和维甲酸（RA）联合对其原癌基因c－myc和c－fos表达的影响。结果表明：硒和RA在第一和第二天都可使c－myc基因表达下降，以后逐渐上升到对照组或稍高水平。联合可使c－myc表达显著下降，强于两单独作用。硒和pA在第三至第四天均可使c－fos表达明显升高，第四天5．8ｕmol／LNa2SeO3和0．1ｕmol／LRA以及联合组c－fos表达水平分别为对照组的2．8，3．7和5．4倍。HL-60 cell line was used to study the combined effects of sodium selenite and all transretinoic acid (RA) on c-myc and c-fos oncogenes expression,Slot blot analysis domonstratedthat 1-2 days after treatment by Na2SeO3 and RA cells showed decreased expression of cmyc, but further treatment slightly increased expression of c-myc gene. Combined treatmentdecreased expression of c-myc more significantly. Both Na2SeO3 and RA showed high expression of c-fos after 3-4 days. 4 days after treatment with 5.8μmol/L Na2SeO3, 0.1μmol/LRA and combined treatment, the expression of c-fos were 2.8, 3.7 and 5.4 times that ofcontrol respectively.

关 键 词：亚硒酸钠 维甲酸 HL-60细胞 白血病 药物疗法 

分 类 号：R733.705[医药卫生—肿瘤]