缺氧诱导因子1α碱性成纤维细胞生长因子在大肠腺癌组织中的表达  

Expression of hypoxia inducible factor 1α and basic fibroblast growth factor in the tissues of colorectal adenocarcinoma

作　　者：韦良宏[1] 陈凤坤[1] 李淑德[2] 谭宗群[1] 陈海东[1] 

机构地区：[1]广西钦州市第一人民医院消化内科,535000 [2]上海长海医院消化内科

出　　处：《中华生物医学工程杂志》2008年第6期432-435,共4页Chinese Journal of Biomedical Engineering

基　　金：广西钦州市科学研究与技术开发计划项目（20062702）

摘　　要：目的观察缺氧诱导因子1α（HIF—1α）和碱性成纤维细胞生长因子（BFGF）在大肠腺癌组织中的表达，探讨其表达与大肠腺癌生物学行为关系。方法采用免疫组织化学染色检测手术切除的大肠腺癌组织（n=60）、癌旁组织（n=60）和正常大肠黏膜组织（n=20）中HIF-1α和BFGF的表达。结果HIF-1α和BFGF在大肠腺癌组织中表达阳性率[61．7％（37／60），65．0％（39／60）]较癌旁组织[10．0％（6／60），13．3％（8／60）]明显升高（P〈0．05）。正常肠黏膜组织中未检出HIF-1α和BFGF表达。HIF—1α和BFGF在大肠腺癌组织中表达与患者性别、年龄、肿瘤大小、肿瘤位置和分化程度无关（P〉0．05）；与肿瘤Dukes分期有关（P〈0．05）。HIF—1α和BFGF在DukesA、B期（n=36）表达阳性率分别为47．2％和52．7％，在DukesC、D期（n=24）分别为83．3％和83．3％（P〈0．05）。大肠腺癌组织中HIF—1α表达与BFGF表达呈正相关（r=-0．4276，P〈0．01）。结论大肠腺癌组织中HIF—1α和BFGF表达水平上调，可能与大肠腺癌的预后有关。HIF—1α和BFGF有协同作用，共同促进大肠腺癌的发生。Objective To observe the expression of hypoxia inducible factor 1α（HIF-1α） and basic fibroblast growth factor （BFGF） in the tissues of colorectal adenocarcinoma and analyze the relationship between expression of two factors and biological behavior of colorectal adenocarcinoma. Methods The samples of colorectal adenocarcinoma （n=60） and para-adenocarcinoma （n=60） were taken from surgical resection patients and normal colorectal tissues （n=20） from patients with irritable bowel syndrome. The expression of HIF- 1α and BFGF were detected by immunohistochemical staining （ SP method）. Results Positive rates of HIF-1α and BFGF in colorectal adenocarcinoma tissues were 61.7% and 65.0%, and in para- adenocarcinoma tissues were 10.0% and 13.3% （P〈0.05, respectively）. HIF-1α and BFGF were not detected in normal intestinal mucosa. There was no association of HIF-1α and BFGF expression in colorectal adenocarcinoma tissues to the gender, age, tumor size, tumor position and differentiation （P〉0.05）. Expression of these two factors was associated with Dukes stages （P〈0.05）. Positive rates of HIF- 1α and BFGF in colorectal adenocarcinoma tissues of Dukes A and B stage were 47.2% and 52.7%, respectively. Positive rates of HIF-1α and BFGF in colorectal adenocarcinoma tissues of Dukes C and D stage were 83.3% and 83.3%, respectively. There was a positive correlation between HIF-1α and BFGF in expression colorectal adenocarcinoma （r=0.4276, P〈0.01）. Conclusion The enhanced expression of HIF- 1 α and BFGF in colorectal adenocarcinoma tissues may be associated with the prognosis of the patients with colorectal adenocarcinoma. HIF - 1 α and BFGF may participate synergistically in the carcinogenesis of colorectal adenocarcinoma.

关 键 词：结直肠肿瘤 缺氧诱导因子1Α 碱性成纤维细胞生长因子 肠易激综合征 DUKES分期 免疫组织化学染色 

分 类 号：R735.34[医药卫生—肿瘤]