APP17肽对缺氧缺血性脑病新生鼠皮质区GAP-43表达的影响  

Effects of APP17-mer peptide on expression of GAP-43 in the cortical area of hypoxic ischemic encephalopathy rats

作　　者：李慧玲[1] 梅梅[1] 刘园园[1] 刘冬焱[1] 顾镜月[1] 

机构地区：[1]佳木斯大学第一附属医院儿一科,黑龙江佳木斯154003

出　　处：《黑龙江医药科学》2009年第3期14-15,共2页Heilongjiang Medicine and Pharmacy

摘　　要：目的:观察APP(β-amyloid precursop protein,APP)17肽(APP695-319-335)肽段对缺氧缺血性脑病新生鼠皮质区GAP-43表达的影响。方法:将生后7d的新生大鼠随机分为假手术组、脑病组和APP17肽治疗组。采用一侧颈动脉结扎,然后放在缺氧仓内2h的方法制备缺氧缺血性脑病模型。应用免疫组化方法观察各组大鼠不同时期脑皮质GAP-43表达的变化。结果:免疫组化结果显示,脑病组较假手术组小鼠皮质区GAP-43免疫阳性细胞增加(P<0.01),治疗组较脑病组GAP-43免疫阳性细胞明显增多(P<0.01)。结论:缺氧缺血性脑损伤后,皮质区GAP-43表达和合成增加,GAP-43的表达增加可能与神经元再生和轴突重塑有关,是脑缺氧缺血后神经细胞内源性代偿机制之一。APP17肽可能通过调节GAP-43的表达来增加神经细胞之间的突触联系,促进神经的生长起到营养神经的作用,从而改善缺氧缺血性脑病小鼠的预后。Objective：To study the effects of APP17--mer peptide on the expression of GAP--43 in the cortical area of hypoxic ischemic encephalopathy rats. Methods：The Wistar rats,aged 7 days, were derided into Sham--operation group,operation group and APP17 group, respectively. HIBD model was formed by adopting the permanent occlusion of one side common carotid artery in Wistar rat,and then put them into a utensil for 2 hours. The GAP--43 expression was assayed by immunohistochemical staining. Results：In hypoxic ischemic en- cephalopathy model, the expression of GAP--43 proteins was increased compared with the sham--operation group （P 〈 0. 01）. The expression of GAP--43 in APP17--mer peptide group was obviously increased compared with the hypoxic ischemic encephalopathy model. Conclusions：The up--regulation expression of GAP-- 43 may be involved in the axonalplasticity and regeneration of neuro--cytes, which is one of the endogenous compensatory mechanisms after hypoxic ischemic encephalopathy. APP17- mer peptide may promote the growth of nerves and enhance the association between synapses, possibly by regulating GAP--43 levels,suggesting that APP17--mer peptide can improve prognosis of hypoxic ischemic encephalopathy rats.

关 键 词：缺氧缺血性脑病 皮质 神经生长相关蛋白-43 神经营养 突触 

分 类 号：R742[医药卫生—神经病学与精神病学] R516[医药卫生—临床医学]