乌拉尔甘草内生真菌拮抗菌株的筛选和鉴定  

Screening and Identification of Antimicrobe Activity of Endophytic Fungus in Glycyrrhiza uralensis

作　　者：王丽[1] 刘磊[1] 韩素贞[1] 

机构地区：[1]首都师范大学生命科学学院,北京100048

出　　处：《生物技术通报》2009年第6期155-158,共4页Biotechnology Bulletin

基　　金：北京市教委基金(KM200510082008)

摘　　要：从乌拉尔甘草样品的根、茎中共分离出54株内生真菌,统计发现从根中分离得到的内生真菌的种类比茎中多。抑菌实验共得到20株活性菌株,其中编号为dG9-1的菌株对多种病原菌有抗菌活性。通过进一步对其培养特征,显微形态特征观察以及ITS序列测定和系统发育树构建与分析,发现该菌与灰黄青霉(Penicillium griseofulvum)的亲缘关系最接近,相似性为99.46%。Fifty-four endophytic fungus were isolated from roots, stems of Glycyrrhiza uralensis. Twenty strains were obtained by the inhibitory activity screening and dGg-1 had the best antimicrobial function among them. Through colony characteristics and morpho- logical characters by microscopy and ITS sequence, analysis results showed that the strain dG9-1 was similar to Penicillium griseofulvum, and the similarities was 99. 46%.

关 键 词：乌拉尔甘草 内生真菌 抑菌活性 鉴定 ITS序列 

分 类 号：S476[农业科学—农业昆虫与害虫防治]