5-Aza-CdR对人肾癌OS-RC-2细胞生长及γ-连环蛋白表达的影响  

Effects of 5-Aza-CdR on proliferation of human renal carcinoma line OS-RC-2 and expression of γ-catenin protein

作　　者：陶美满[1] 孙浩[2] 田福起[1] 马克钧[2] 郭涛[2] 潘鹏[2] 徐强[2] 

机构地区：[1]江苏大学临床医学院,江苏镇江212001 [2]江苏大学附属医院泌尿外科,江苏镇江212001

出　　处：《江苏大学学报（医学版）》2009年第3期253-255,共3页Journal of Jiangsu University：Medicine Edition

基　　金：江苏大学临床医学科技发展基金项目(JLY20050015)

摘　　要：目的:研究甲基化抑制剂5-氮杂-2′-脱氧胞苷(5-Aza-CdR)对人肾癌OS-RC-2细胞增殖、凋亡及γ-连环蛋白(γ-catenin)表达的影响。方法:使用不同浓度(10-7,10-6,10-5,10-4mol/L)的特异性DNA甲基转移酶抑制剂5-Aza-CdR处理OS-RC-2肾癌细胞株。MTT检测5-Aza-CdR对肾癌细胞株OS-RC-2的生长抑制作用。流式细胞仪检测5-Aza-CdR对OS-RC-2肾癌细胞株凋亡的影响。细胞免疫化学检测5-Aza-CdR处理OS-RC-2肾癌细胞株后γ-catenin表达的变化。结果:5-Aza-CdR明显抑制肾癌细胞的增殖,促进其凋亡,且与药物浓度及作用时间有关。药物处理后γ-连环蛋白表达增加。结论:γ-catenin蛋白表达受甲基化的抑制,提示5-Aza-CdR可使γ-catenin基因去甲基化而抑制肾癌OS-RC-2细胞株增殖,并促进其凋亡。Objective： To investigate the effect of methylation inhibitor 5-Aza-2-deoxycytidine on the growth of human renal cancer cell line OS-RC-2 and γ-catenin expression. Methods： Human renal carcino- ma OS-RC-2 cells were treated with different concentrations of 5-Aza-CdR（ 10^-7,10^-6,10^-5,10^-4 tool/L） respectively. Then the growth rate of the cells was detected by MTT assay. The apoptosis was analyzed by flow cytometry. The immuneocytochemistry was used to detect γ-catenin levels in the cell line before and after treatment with 5-Aza-CdR. Results： 5-Aza-CdR can inhibit the growth of OS-RC-2 cells, and the apoptotic rate was also increased after 5-Aza-CdR treatment. γ-catenin expression levels in OS-RC-2 cells were increased after 5-Aza-CdR treatment. Conclusion： γ-catenin expression levels were inhibited by methyla- tion, 5-Aza-CdR can inhibit the growth, and promote the apoptosis of OS-RC-2 cells by inducing demethyla- tion in the promoter region of γ-catenin gene.

关 键 词：5-氮杂-2′-脱氧胞苷 肾癌 Γ-连环蛋白 细胞凋亡 甲基化 

分 类 号：R73-35[医药卫生—肿瘤]