嗜中性活性肽2(NAP-2)在人肝癌细胞株和肝癌组织的表达  

Expressions of NAP-2 protein in human hepatoma cell line SMCC-7721 and hepatocellular carcinoma tissues

作　　者：蒋作祎[1] 钟艳平[2] 覃健[1] 何敏[2] 张志勇[1] 

机构地区：[1]广西医科大学公共卫生学院,广西南宁530021 [2]广西医科大学医学科学实验中心,广西南宁530021

出　　处：《现代肿瘤医学》2009年第6期1122-1124,共3页Journal of Modern Oncology

基　　金：广西科学基金项目(桂科能05112001-4A;桂科能0630006-5E;桂科攻0592007-1C)

摘　　要：目的:探讨NAP-2在人肝癌细胞株SMCC-7721和人肝正常细胞株HL-7702、肝癌组织及其癌旁组织表达的差异性。方法:采用免疫细胞化学法(Immunocytochemistry,ICC)检测人肝癌细胞株SMCC-7721和人肝正常细胞株HL-7702中NAP-2蛋白的表达,免疫组织化学法(Immunohistochemistry,IHC)检测30例肝癌组织及其癌旁组织中NAP-2蛋白的表达。结果:NAP-2在人肝癌细胞株SMCC-7721表达率为100%,人肝正常细胞株HL-7702无表达。在肝癌组织中强阳性表达率为63.33%,癌旁组织无强阳性表达;经统计学分析,两者NAP-2的表达呈显著差异(P<0.01)。结论:NAP-2在肝癌发生、发展过程中可能起着一定作用。Objective： To detect and analyze the expression of NAP - 2 in SMCC - 7721 and HL - 7702, hepatocellular carcinoma and its adjacent tissues. Methods：Immunocytochemical staining and immunohistochemical method were used to detect expressions of NAP -2 protein in SMCC -7721 and HL -7702,30 hepatocellular carcinoma tissues and their adjacent tissues ,respectively. Results：In SMCC -7721, the NAP -2 protein positive rates was 100%, there was no NAP - 2 protein expression in HL - 7702. In 30 hepatocellular carcinoma tissues, NAP - 2 protein strong positive rate was 63.33 % , but no strong expression in HCC adjacent tissues （P 〈 0.01 ）. Conclusion：NAP -2 protein may be related with the occurrence, development and progress of hepatocellular carcinoma.

关 键 词：肝癌 嗜中性活性肽-2 免疫细胞化学 免疫组织化学 

分 类 号：R735.7[医药卫生—肿瘤]