TGF-β上调在Wistar大鼠抑制EAMG发生中的重要作用  

THE UPREGULATION OF TGFβ PLAYS AN IMPORTANT ROLE IN SUPPRESSION OF EXPERIMENTAL AUTOIMMUNE MYASTHENIA GRAVIS IN WISTAR RATS

作　　者：梁丽云[1,2,3] 马存根[1,2,3] 张光先 肖保国[1,2,3] 

机构地区：[1]大同医学专科学校 [2]河南医科大学附属一院神经科 [3]上海医科大学华山医院

出　　处：《中国免疫学杂志》1998年第3期187-189,共3页Chinese Journal of Immunology

摘　　要：用ＡＣｈＲ加ＣＦＡ免疫大鼠后，Ｌｅｗｉｓ大鼠出现典型的临床肌无力，而ＷｉｓｔａｒＦｕｒｔｈ（Ｗ．Ｆ）大鼠不出现任何症状。为阐明Ｗ．Ｆ大鼠对ＡＣｈＲ耐受的机制，检测了表达ＩＦＮ－γ、ＩＬ－４和ＴＧＦ－βｍＲＮＡ的ＭＮＣ和肌肉ＡＣｈＲ。结果表明，Ｗ．Ｆ大鼠免疫后第５、７周ＰＩＬＮ中ＡＣｈＲ诱导的ＩＦＮ－γｍＲＮＡ表达细胞数比Ｌｅｗｉｓ大鼠低，ＴＧＦ－βｍＲＮＡ表达细胞数比Ｌｅｗｉｓ大鼠高，肌肉ＡＣｈＲ含量比Ｌｅｗｉｓ大鼠高。提示ＩＦＮ－γ和ＴＧＦ－β与ＥＡＭＧ的发生有关，ＴＧＦ－β上调可抑制ＩＦＮ－γｍＲＮＡ表达，减少肌肉ＡＣｈＲ丢失。fter immunization with AChR plus CFA, Lewis rats displayed the typical signs of EAMG, while Wistar Furth(WF) rats did not present any signs of EAMG. To clarify the mechanism of WF rats tolerance to AChR and the effect of cytokines in tolerance, in situ hybridization with radiolabelled cDNA oligonucleotide probes was adopted to enumerate mononuclear cells(MNC) expressing mRNA for interferonγ(IFNγ), interleukin4(IL4) and transforming growth factorβ, radioimmunoassay was used to examine the muscle AChR content. The results showed that WF rats had lower numbers of AChRinduced IFNγ mRNA expressing cells and higher numbers of AChRinduced TGFβ mRNA expressing cells in MNC isolated from popliteal and inguinal lymph nodes(PILN) compared to Lewis rats after immunization at the fifth week and the seventh week. Muscle AChR content was higher in WF rats than that in Lewis rats. The results suggested that IFNγ and TGFβ were involved in the development of EAMG; TGFβ upregulation could inhibit IFNγ mRNA expression and decrease muscle AChR loss, further prevent and suppress the occurrence of EAMG.

关 键 词：EAMG 重症肌无力 TGF-Β 耐受 

分 类 号：R746.1[医药卫生—神经病学与精神病学]