兔VX_2舌鳞癌移植瘤模型的建立及生物学特性观察  

Establishment of transplanted model of VX_2 tongue carcinoma in rabbits and observation of their major biological features

作　　者：廖贵清[1] 田军[1] 刘海潮[1] 杜日昌 李传真[1] 李清[1] 王敬旭[1] 李国永[1] 

机构地区：[1]中山大学附属口腔医院口腔颌面外科,广东广州510055 [2]广东省韶关市第一人民医院病理科,广东韶关512000

出　　处：《中国口腔颌面外科杂志》2009年第3期244-248,共5页China Journal of Oral and Maxillofacial Surgery

基　　金：国家自然科学基金(30171019);广东省自然科学基金(990089)~~

摘　　要：目的:建立可供实验研究的稳定的兔VX2舌鳞癌移植瘤模型,观察其主要生物学特性。方法:制备VX2肿瘤荷瘤种兔后,将荷瘤种兔新鲜鳞癌组织块移植于24只新西兰大白兔的舌侧缘中1/3黏膜下,制成兔VX2舌鳞癌移植瘤模型。荷瘤动物随机分为2组,第1组(n=6)不同时期分批处死,进行大体观察、组织病理及电镜检查;第2组(n=14)作为对照组,观察荷瘤动物的自然生存期。结果:成瘤率为83.3%(20/24);移植瘤的组织学特征与移植于兔其他部位的VX2肿瘤基本一致,在舌体内浸润性生长并向周围侵袭、扩散,符合中分化鳞状细胞癌的特征。结论:成功建立了兔VX2舌鳞癌移植瘤模型。该模型成瘤率高,潜伏期短,生长迅速,生物学特性稳定,易于复制,接近人舌鳞癌的临床特征,较客观地反映了舌癌的生物学行为,为舌鳞癌的研究提供了较理想的大型动物模型。PURPOSE： To establish a stable large animal transplanted model of VX2 carcinoma in rabbit tongue and observe its major biological features. METHODS： After establishment of VX2 tumor-bearing rabbits, the carcinoma tissues were transplanted into the middle third of the lateral border of the tongues in twenty-four New-Zealand white rabbits submucosally to establish transplanted tumor model. The animals were randomly divided into two groups. Rabbits in groupl（n=6） were randomly sacrificed at day 3, 7, 14, 21, 28, and 35 after implantation, respectively, Tumor biological features were observed maeroseopieally and pathohistologieally. The natural survival time of tumor-beating rabbits were observed in group 2 （n=14）. RESULTS： The tumor-loading rate was 83.3%（20/24）. Histopathologieal examination indicated that the transplanted tumour grew infihratively and invasively at the lingual recipient sites, which was similar to the biological properties of VX2 transplanted squamous cell carcinoma in other sites of the rabbits. CONCLUSIONS： We successfully established a transplanted model of VX2 squamous cell carcinoma in rabbit tongues. This model has high tumor-loading rate, steady biological features and rapid growth, which resembles the features of tongue squamous cell carcinoma in human. Therefore, this large animal model is useful for studies on the invasive mechanisms, treatment, and prevention of human carcinoma of tongue.

关 键 词：舌肿瘤 VX2肿瘤细胞株 动物模型 兔 

分 类 号：R739.8[医药卫生—肿瘤]