鱼油、豆油对饲低硒粮大鼠机体抗氧化酶和血浆酶活性的影响  

Influence of fish oil and soybean oil on the activities of antioxidantenzyme and plasma enzymes in rates fed on selenium deficient cereals

作　　者：胡秀丽[1] 卜凤泉[1] 姜熙罗[1] 安汝国 

机构地区：[1]白求恩医科大学应用基础医学研究所

出　　处：《白求恩医科大学学报》1998年第3期239-241,共3页Journal of Norman Bethune University of Medical Science

基　　金：国家八.五攻关项目

摘　　要：目的：观察鱼油和豆油对饲低硒及补硒粮喂养大鼠血谷胱甘肽过氧化物酶（ＧＳＨ－Ｐｘ）及血浆酶活力的影响。方法：ＧＳＨ－Ｐｘ采用ＤＴＮＢ法而血浆酶采用自动生化分析仪测定。结果：补加鱼油和豆油（５０ｇ／ｋｇ）饲料喂养大鼠４周，血中ＧＳＨ－Ｐｘ活力均明显降低；豆油组血浆中ＡＬＰ显著增加，ＧＯＴ、ＧＰＴ、α－ＨＢＤＨ有升高趋势；鱼油组ＧＰＴ显著增加。补硒后ＧＳＨ－Ｐｘ活力均显著升高，豆油中血浆酶活性有不同程度改善，鱼油组变化不显著。结论：多不饱和脂肪酸负荷能够降低动物体内抗氧化能力。鱼油（ω－３）。Objective:The effect of fish oil (FO) and soybean oil (SO) on activities of blood glutathione peroxidase (GSH-Px) and plasma enzymes of rats fed on selenium (Se) deficient (0.011 μg/g Se) or Sesupplemented (0.1 μg/g Se) diets was investigated.Methods:The GSH-Px activity was determined by DTNB and plasma enzymes by automatic biochemistry analyzer.Rats were maintained on the Se deficient or Sesupplemented diets for 4 weeks,and then on the diets supplemented with FO or SO (50 g/kg) for 4 weeks.Results: The blood GSH-Px activity was significantly decreased in both FO and SOsupplemented groups.In SO group,activities of GOT,GPT and α-HBDH in plasma tended to increase and activity of ALP increased obviously,while in FO group only GPT activity increased markedly.After Se was supplemented,the GSH-Px activity increased significantly.In SO group,the changes of plasma enzymes were corrected by Se supplement,but in FO group they can not.Conclusion:These results prove that polyunsaturates fatty acids (PUFAs) overload can decrease the antioxidant ability of rats fed on the Se deficient diets.The influence of FO on plasma enzyme levels is different from that of SO.

关 键 词：鱼油 豆油 GSH-PX 血浆酶 硒 ALP 克山病 

分 类 号：R542.302[医药卫生—心血管疾病]