盐酸小檗碱对大鼠牙周组织中白细胞介素-6表达的影响  

Effect of Berberine Hydrochloride on Expression of Interleukin-6 in Periodontal Tissues in Rats

作　　者：张小恒 余占海[2] 杨倩[2] 张国英[2] 杜建东[2] 何福德[2] 

机构地区：[1]甘肃省甘谷县人民医院,甘肃甘谷741200 [2]兰州大学口腔医学院,甘肃兰州730000

出　　处：《上海中医药大学学报》2009年第3期64-66,共3页Academic Journal of Shanghai University of Traditional Chinese Medicine

基　　金：甘肃省科技攻关基金资助项目(2GS064-A43-020-34);兰州市科技攻关基金资助项目(06-1-1)

摘　　要：目的:探讨盐酸小檗碱对大鼠实验性牙周炎模型牙周组织中白细胞介素-6表达的影响。方法:将Wistar大鼠随机分为正常组和造模组,复制牙周炎动物模型,并对治疗组灌服盐酸小檗碱0.6 mg.kg-1.d-1。动态观察牙周组织形态变化,并采用免疫组织化学SABC方法检测牙周组织中IL-6水平的变化。结果:牙周炎模型组牙周组织中IL-6表达明显高于正常组(P<0.05);用药后第1、2、3、4周各时间段牙周炎治疗组牙周组织中IL-6表达明显低于模型组(P<0.05),但治疗组各时间段之间IL-6表达无显著差异。结论:大鼠实验性牙周炎模型牙周组织中IL-6表达水平较正常组明显升高,应用盐酸小檗碱能抑制实验性牙周炎模型牙周组织中IL-6的表达。To study the effects of berherine hydrochloride on the expressions of Interleukin-6 in periodontal tissues in rat periodontitis model. Methods： Wistar rats were randomized into normal group and experimental pefiodontitis model group, and the periodontitis animal model was replicated. The rats in treatment group were administered with berberine hydrochloride （ 0.6 mg~ kg-~ ~ d~ ）. Detect the general conditions and pathological changes of rat periodontal tissues. And immunohistoehemical staining was conducted to determine the expressions of IL-6 in rats periodontal tissues. Results ： The level of IL-6 in periodontal tissues of rats in model group was much higher than that in the normal group （P 〈 0.05 ）. The level of IL-6 in the treatment group was much lower than that in the control group in 1 st, 2nd, 3rd and 4th weeks （P 〈 0.05 ）, but there was no significant difference among the various time quantum in the treatment group. Conclusion： The level of IL-6 in rats periodontitis group is much higher than that in the normal group, and the berberine hydroehloride can reduce the IL-6 level in periodontal tissues in rats.

关 键 词：牙周炎 盐酸小檗碱 白细胞介素-6 动物实验 

分 类 号：R781.4[医药卫生—口腔医学] R285.5[医药卫生—临床医学]