大肠癌外周血及淋巴结癌胚抗原mRNA表达  

Expression and clinical significance of CEA mRNA in patients with colon cancer

作　　者：徐兰[1] 李晓霞[2] 

机构地区：[1]辽宁省肿瘤医院检验科,沈阳110042 [2]中国医科大学附属第四医院

出　　处：《中国公共卫生》2009年第6期729-730,共2页Chinese Journal of Public Health

基　　金：辽宁省自然科学基金(20042044)

摘　　要：目的了解大肠癌患者不同临床分期外周血及淋巴结中癌胚抗原信使核糖核酸(CEA mRNA)相对表达量,为大肠癌术前诊断及分期提供依据。方法采用实时荧光定量RT-PCR技术检测40例大肠癌患者和10名健康对照者外周血及淋巴结中CEA mRNA的相对表达量。结果40例大肠癌患者手术前外周血CEA mRNA表达阳性率为87.5%,与正常对照组(10人无1例阳性)比较,差异有统计学意义(P<0.01);淋巴结A+B期阳性率15.8%,C+D期阳性率53.3%,差异有统计学意义(χ2=5.4,P<0.05)。C+D期大肠癌患者外周血中CEA mRNA阳性率较A+B期为高,但差异无统计学意义(χ2=0.75,P>0.05)。结论大肠癌患者外周血中CEA mRNA相对表达量高于正常健康者,检测结果有助于大肠癌患者术前辅助诊断;淋巴结CEA mRNA阳性表达与不同临床分期密切相关,可作为大肠癌患者临床分期的参考。Objective To understand the CEA mRNA relative expression in the peripheral blood and lymph nodes in patients with different stages of colon cancer and its use for preoperative diagnosis and staging of the colon cancer. Methods Real-time RT-PCR was adopted to detect the CEA mRNA expression in lymph node and in peripheral blood of 10 normal individuals and 40 colon cancer patients. Results CEA mRNA positive rate was 87.5% in peripheral blood in 40 preopera- tive patients. No CEA mRNA was detected in 10 normal individuals,and the difference was significant . The positive CEA mRNA expression in the lymph nodes of A ＋ B stage cancer was 15.8% ,and C ＋ D stage was 53.3%. Clinical stage was closely related to the positive CEA mRNA expression and the difference was significant（ X2 = 5.4 ,P 〈 0.05 ）. The positive CEA mRNA expression in the peripheral blood of C ＋ D stage colon cancer patients was higher than that of A ＋ B stage, but the difference was not significant（ x2 = 0.75 ,P 〉 0.05 ）. Conclusion The CEA mRNA expression in the peripheral blood of patients with colon cancer is higher than that of normal individuals. The results is useful for the preperative diagnosis of colon cancer. Positive CEA mRNA expression in the lymph nodes is related to different clinical staging and can be used as the reference of clinical stage of colon cancer.

关 键 词：荧光定量PCR 癌胚抗原信使核糖核酸(CEAmRNA) 外周血 淋巴结 

分 类 号：R735.34[医药卫生—肿瘤]