阳离子交换型连续床用超大孔晶胶的制备  

Preparation of Supermacroporous Cryogel Used in Cation-Exchange Monolith

作　　者：王良华[1,2] 陈芳[2] 贠军贤[2] 姚克俭[2] 姚善泾[1] 

机构地区：[1]浙江大学化学工程与生物工程学系,浙江杭州310027 [2]浙江工业大学化学工程与材料学院,绿色化学合成国家重点实验室培育基地,浙江杭州310032

出　　处：《高校化学工程学报》2009年第3期480-485,共6页Journal of Chemical Engineering of Chinese Universities

基　　金：浙江省自然科学基金资助项目(Y405054)

摘　　要：采用原位接枝法,将3-烯丙氧基-2-羟基-1-丙磺酸钠(AHPSA)接枝到聚丙烯酰胺基晶胶基质孔隙内表面,制备得到了阳离子交换型连续床用的带磺酸基超大孔晶胶介质。通过脉冲示踪法测量停留时间分布(RTD),得到了晶胶介质的理论等板高度(HETP)。通过测量一定压差下流经晶胶床柱的液量,得到晶胶介质的渗透率。用溶菌酶作为模型蛋白测量晶胶介质的吸附容量。考察了单体浓度、接枝反应时间等反应条件对晶胶介质的HETP、渗透率、蛋白质吸附容量等性能的影响。结果表明:接枝AHPSA的阳离子交换晶胶介质的HETP基本不受接枝反应时间和单体浓度的影响,其渗透率随接枝反应时间增大略有减小,其对溶菌酶的吸附容量与接枝单体的浓度成正比,受接枝反应时间的影响较小。A novel supermacroporous cryogel with sulfo binding groups used in cation-exchange monolith was prepared by in-situ grafting of 3-allyloxy-2-hydroxy-l-propanesulfonic acid sodium salt （AHPSA） onto the pore surfaces of polyacrylamide-based cryogel. The height equivalent to a theoretical plate （HETP） of the cryogel beds was obtained by measuring residence time distributions （RTDs） with tracer pulse-response method. The hydraulic permeability was calculated by measuring the flow rate of deionized water passing through the cryogel bed at fixed hydrostatic pressure drops. Lysozyme was employed as the model protein to reveal the protein binding capacity of the cryogel bed. The effects of AHPSA concentration and reaction time on HETP, hydraulic permeability and protein binding capacity were investigated. The results show that the HETP is not influenced by the graft reaction time and monomer concentration, while the permeability slightly decreases with the increase of graft reaction time. The binding capacity of lysozyme increases linearly with the increase of the concentration of AHPSA and has no obvious change under different graft reaction times.

关 键 词：生物分离 超大孔连续床 原位接枝 晶胶 阳离子交换 

分 类 号：O648.17[理学—物理化学] TQ028.8[理学—化学]