多酚氧化酶抑制剂对蝴蝶兰叶外植体褐变的影响  

Influences of PPO Inhibitors on Browning in Phalaenopsis Explants

作　　者：陈冬茵[1] 赖艳艳[1] 许传俊[1,2] 李玲[1] 

机构地区：[1]华南师范大学生命科学学院,广东省植物发育生物工程重点实验室,广东广州510631 [2]福建省亚热带植物研究所,福建厦门361006

出　　处：《亚热带植物科学》2009年第2期15-18,共4页Subtropical Plant Science

基　　金：国家大学生创新性实验计划项目(081057411);华南师范大学2008年度大学生创新性实验项目资助

摘　　要：将多酚氧化酶(PPO)抑制剂添加到酶反应液中,抗坏血酸和半胱氨酸在0.5mmol/L就完全抑制蝴蝶兰PPO活性。300mg/L柠檬酸和100～200mg/L亚硫酸氢钠分别添加到培养基中,可使蝴蝶兰外植体褐变程度降低;采用抑制剂浸泡处理外植体,对外植体褐变抑制效果最好的为50mg/L抗坏血酸,外植体在褐变发生前PPO活性低于对照。The effects of Polyphenol oxidase （PPO） activity inhibitors supplemented to reaction buffer at different concentrations showed that ascorbate acid and Cys complete inhibited PPO activity at 0.5 mmol/L. Adding PPO activity inhibitors to media at different concentrations, the results showed that 300 mg/L citric acid and 100-200mg/L NaHSO3 could reduce the browning of Phalaenopsis explants. Soaking explants with PPO activity inhibitors, it showed that 50 mg/L ascorbate acid gave the best result for browning inhibition and PPO activity was lower than that of control before explants browning happened.

关 键 词：蝴蝶兰 外植体 褐变 多酚氧化酶 抑制剂 

分 类 号：S682.31[农业科学—观赏园艺] Q943.1[农业科学—园艺学]