实验性牙移动大鼠牙周组织环氧化酶2的表达及意义  

Expression of cyclooxygenase-2 in rat periodontal tissues during experimental tooth movement and its significance

作　　者：侯旭[1] 孙新华[1] 

机构地区：[1]吉林大学口腔医院正畸科,吉林长春130041

出　　处：《吉林大学学报（医学版）》2009年第3期423-426,F0002,共5页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金(面上项目)种子基金资助课题(419070401431)

摘　　要：目的:研究大鼠实验性牙移动过程中环氧化酶2(COX-2)在牙周组织中的表达变化,探讨COX-2与正畸牙移动过程中血管改建的关系。方法:35只Wistar大鼠,随机分为7组:正常组和实验性牙移动模型1、3、5、7、14和21 d组,每组5只。实验各组动物于双侧上颌第一磨牙至切牙间拴结NiTi螺簧,以上颌中切牙为支抗,0.294N的拉力牵引第一磨牙向近中移动。牙根近中侧牙周组织为压力区,牙根远中侧牙周组织为张力区。以上颌第一磨牙为中心、近远中方向进行组织切片,对牙周组织切片行HE染色与COX-2免疫组织化学染色,光镜下行形态学观察,对COX-2表达的灰度积分进行图像分析。结果:正常组大鼠牙周组织中COX-2仅有少量表达(134.75±5.25);实验1 d组压力区牙周组织中COX-2的表达(147.73±3.27)高于正常组(P<0.05),至5 d达高峰(154.32±9.54);实验3 d组张力区牙周组织中COX-2表达(139.16±5.01)高于正常组(P<0.05),至7 d组达高峰(159.46±7.48);实验21 d组压力区和张力区牙周组织中COX-2表达与正常组比较差异均无显著性(P>0.05)。结论:在实验性牙移动过程中大鼠牙周组织COX-2的表达增强,提示COX-2可促进正畸牙移动过程中牙周组织的血管改建。Objective To investigate the expression and distribution of cyclooxygenase-2 （COX-2） in periodontal tissues during experimental tooth movement in rats, and study the relationship between COX-2 and vesscular reconstruction in experimental tooth movement process. Methods Thirty-five healthy Wistar rats were divided randomly into 7 groups on average： normal group and experimental groups for 1, 3, 5, 7, 14 and 21 d. A NiTi coil spring with 0.294 N mesial force was connected between first molars of maxillary and the upper incisors. The histological sections were stained with goat anti rat COX-2 antibody, and computer image analysis was used to study the expression of COX-2 in the periodontal tissues of rats. Results Pressure area： compared with normal group （134.75±5.25） the COX-2 expression in 1 d group （147.73±3.27） increased （P〈0.05） after tooth movement and reached the maximum at 5 d （154.32 ± 9.54）. Tension area： compared with normal group, the COX-2 expression in 3 d group （139.16±5.01） increased （P〈0.05） after tooth movement and reached the maximum at 7 d （159.46±7.48）. There was no significant difference of COX-2 expression in pressure area and tension area bewteen 21 d group and normal group （P〉0.05）. Conclusion The expression of COX-2 in periodontal tissues during experimental tooth movement increase, suggesting that COX-2 can promote the vascular reconstruction in periodontal tissues during orthodontic tooth movement.

关 键 词：实验性牙移动 牙周组织 环氧化酶2 免疫组织化学 

分 类 号：R783.5[医药卫生—口腔医学] R-332[医药卫生—临床医学]