胶原-壳聚糖和角膜基质细胞复合膜的构建及其生物相容性  

Construction of compound membrane with corneal stromal cells and collagen-chitosan and its biocompatibility

作　　者：贾卉[1] 王娇[1] 胡源[1] 张媛 张冰[3] 

机构地区：[1]吉林大学第一医院眼科,吉林长春130021 [2]日本Ehime大学眼科,日本790-0925 [3]吉林大学中日联谊医院眼科,吉林长春130033

出　　处：《吉林大学学报（医学版）》2009年第3期440-443,579,共5页Journal of Jilin University：Medicine Edition

基　　金：吉林省科技厅重点科研项目资助课题(20010407))

摘　　要：目的:探讨以胶原-壳聚糖为生物支架构建角膜基质细胞复合膜及其生物相容性。方法:兔和人角膜基质细胞分离培养后种植到胶原-壳聚糖共混膜上,构建成复合膜后移植到异体大耳白兔角膜基质囊袋中,术后1、2和4周时分别于活体进行前节OCT、Cs-4检查和离体组织学及免疫组化检测,观察复合膜中角膜基质细胞生长状态、生物支架对正常角膜细胞的影响及生物膜的降解情况。结果:活体前节OCT、Cs-4检查和离体组织学及免疫组化观察结果显示,兔和人角膜基质细胞在胶原-壳聚糖共混膜上生长良好,移植后复合膜逐渐发生降解,角膜组织未发生坏死和溶解,角膜上皮、基质和内皮细胞形态结构正常。结论:胶原-壳聚糖可作为角膜基质构建的生物支架,角膜共焦显微镜及前节OCT作为新的手段可活体观察构建后的角膜基质生物学活性。Objective To investigate the construction of compound membrane with corneal stromal cells and collagen-chitosan by tissue engineering technique and its biocompatibility. Methods Rabbit and human corneal stromal cells were separated and seeded into collagen-chitosan membrane. The compound membrane was transplanted into rabbit corneal stroma. Then the growth condition of keratocytes, the effect on normal keratocytes and degradation of compound membrane were detected by corneal confocal microscope, anterior OCT and histological and immunohistochemical methods ex vivo 1, 2, 4 weeks after grafting. Results The rabbit and human corneal stromal cells grown well in collagen-chitosan scaffold. The compound membrane degradated gradually after grafting. There was no necrosis and dissolvation. Corneal epithelium, stroma and endothelial cells were all normal. Conclusion Collagen-chitosan can be used as a biological scaffold for construction of corneal stroma. Corneal confocal microscopy and anterior OCT are new methods to observe the biological activity of constructed corneal stroma.

关 键 词：角膜基质细胞 胶原-壳聚糖 组织工程 

分 类 号：Q813[生物学—生物工程] R77[医药卫生—眼科]